•Several Enterovirus 71 (EV-A71) strains bearing single nucleotide mutations were constructed.•The contribution of each mutation to virulence was evaluated.•The nucleotides that contributed to significant reduction in virulence in vitro were selected.•Each mutation was introduced separately into the genome to construct the multiply mutated EV-A71 strain (MMS).•The MMS had low virulence as it elicited low viral RNA, plaque count, VP1 and high TCID50, indicative of a vaccine candidate.
Virus Research – Elsevier
Published: Jun 15, 2017
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