Changes in protein and mRNA expression levels of claudin family after mucosal lesion by intestinal ischemia/reperfusion

Changes in protein and mRNA expression levels of claudin family after mucosal lesion by... Article history: Ischemia/reperfusion (I/R) injury of the intestine is the leading cause of organ dysfunction after restora- Received 4 October 2011 tion of blood flow after diverse events, including shock and intestinal transplantation. I/R injury must be Received in revised form overcome for successful small intestinal transplantation. Tight junctions (TJ) are the most apical compo- 19 December 2011 nent of the intercellular junctional complex in epithelial cells; they establish cell polarity and functioning Accepted 12 January 2012 as major determinants of epithelial barrier function. Among the proteins that comprise TJ, the claudin Available online 20 January 2012 family is thought to play a crucial role in homeostasis in multicellular organisms. Therefore, the aim of this study was to examine the changes in function of TJ and behavior of the claudin family during intesti- Keywords: nal I/R. Wistar/ST rats underwent intestinal ischemia by using the spring scale and surgical suture for In vivo intestinal ischemia/reperfusion 1 h, followed by 24 h of reperfusion. We examined the changes in area under the blood concentration Tight junction curve (AUC) after oral administration of FD-4, which is a paracellular marker, and claudin-1, -2, -4, and Claudin family Paracellular permeability -7 mRNA and protein http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Pharmaceutics Elsevier

Changes in protein and mRNA expression levels of claudin family after mucosal lesion by intestinal ischemia/reperfusion

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Publisher
Elsevier
Copyright
Copyright © 2012 Elsevier B.V.
ISSN
0378-5173
D.O.I.
10.1016/j.ijpharm.2012.01.023
Publisher site
See Article on Publisher Site

Abstract

Article history: Ischemia/reperfusion (I/R) injury of the intestine is the leading cause of organ dysfunction after restora- Received 4 October 2011 tion of blood flow after diverse events, including shock and intestinal transplantation. I/R injury must be Received in revised form overcome for successful small intestinal transplantation. Tight junctions (TJ) are the most apical compo- 19 December 2011 nent of the intercellular junctional complex in epithelial cells; they establish cell polarity and functioning Accepted 12 January 2012 as major determinants of epithelial barrier function. Among the proteins that comprise TJ, the claudin Available online 20 January 2012 family is thought to play a crucial role in homeostasis in multicellular organisms. Therefore, the aim of this study was to examine the changes in function of TJ and behavior of the claudin family during intesti- Keywords: nal I/R. Wistar/ST rats underwent intestinal ischemia by using the spring scale and surgical suture for In vivo intestinal ischemia/reperfusion 1 h, followed by 24 h of reperfusion. We examined the changes in area under the blood concentration Tight junction curve (AUC) after oral administration of FD-4, which is a paracellular marker, and claudin-1, -2, -4, and Claudin family Paracellular permeability -7 mRNA and protein

Journal

International Journal of PharmaceuticsElsevier

Published: Apr 15, 2012

References

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