Cannabinoid modulation of rat pup ultrasonic vocalizations

Cannabinoid modulation of rat pup ultrasonic vocalizations The present study investigated the effects of the cannabinoid receptor agonist CP 55,940 ((−)- cis -3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)- trans -4-(3-hydroxypropyl)cyclohexanol) and the cannabinoid receptor antagonist SR 141716A ( N -(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1 H -pyrazole-3-carboxamide hydrochloride) on ultrasonic vocalizations, body temperature and activity in 11–13-day-old rat pups. Testing occurred in a 5-min session 30 min following drug administration. CP 55,940 produced a dose-dependent decrease in ultrasonic vocalizations, with a 1000-μg/kg dose causing an almost complete inhibition of calls. Doses of 100 and 1000 μg/kg of CP 55,940, but not 10 μg/kg, caused significant hypothermia in the pups and the 1000-μg/kg dose also inhibited activity. The cannabinoid receptor antagonist SR 141716A (20 mg/kg) reversed the effects of 1000 μg/kg CP 55,940 on ultrasonic vocalizations and body temperature, but the benzodiazepine receptor antagonist flumazenil (20 mg/kg), the dopamine D 1 receptor antagonist SCH 23390 (0.5 mg/kg) and the opioid receptor antagonist naloxone (1 mg/kg) did not. When administered alone, SR 141716A (20 mg/kg) increased pup ultrasonic vocalizations without affecting body temperature or activity. These results indicate that cannabinoids modulate ultrasonic vocalization production in rat pups in a manner that is independent of hypothermia. The increase in ultrasonic vocalizations produced by SR 141716A is one of the first reported behavioural effects of this drug and suggests that the endogenous cannabinoid ligand anandamide may be involved in the regulation of ultrasonic vocalizations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Journal of Pharmacology Elsevier

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Publisher
Elsevier
Copyright
Copyright © 1996 Elsevier Ltd
ISSN
0014-2999
DOI
10.1016/0014-2999(96)00511-0
Publisher site
See Article on Publisher Site

Abstract

The present study investigated the effects of the cannabinoid receptor agonist CP 55,940 ((−)- cis -3-(2-hydroxy-4-(1,1-dimethylheptyl)phenyl)- trans -4-(3-hydroxypropyl)cyclohexanol) and the cannabinoid receptor antagonist SR 141716A ( N -(piperidin-1-yl)-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1 H -pyrazole-3-carboxamide hydrochloride) on ultrasonic vocalizations, body temperature and activity in 11–13-day-old rat pups. Testing occurred in a 5-min session 30 min following drug administration. CP 55,940 produced a dose-dependent decrease in ultrasonic vocalizations, with a 1000-μg/kg dose causing an almost complete inhibition of calls. Doses of 100 and 1000 μg/kg of CP 55,940, but not 10 μg/kg, caused significant hypothermia in the pups and the 1000-μg/kg dose also inhibited activity. The cannabinoid receptor antagonist SR 141716A (20 mg/kg) reversed the effects of 1000 μg/kg CP 55,940 on ultrasonic vocalizations and body temperature, but the benzodiazepine receptor antagonist flumazenil (20 mg/kg), the dopamine D 1 receptor antagonist SCH 23390 (0.5 mg/kg) and the opioid receptor antagonist naloxone (1 mg/kg) did not. When administered alone, SR 141716A (20 mg/kg) increased pup ultrasonic vocalizations without affecting body temperature or activity. These results indicate that cannabinoids modulate ultrasonic vocalization production in rat pups in a manner that is independent of hypothermia. The increase in ultrasonic vocalizations produced by SR 141716A is one of the first reported behavioural effects of this drug and suggests that the endogenous cannabinoid ligand anandamide may be involved in the regulation of ultrasonic vocalizations.

Journal

European Journal of PharmacologyElsevier

Published: Oct 10, 1996

References

  • Ultrasonic vocalizations by rat pups: the primary importance of ambient temperature and the thermal significance of contact comfort
    Blumberg, M.S.; Efimova, I.V.; Alberts, J.R.
  • Prevention by the cannabinoid antagonist
    Collins, D.R.; Pertwee, R.G.; Davies, S.N.
  • Reluctance of rats to drink hashish suspensions: free-choice and forced consumption, and the effects of hypothalamic stimulation
    Corocoran, M.E.; Amit, Z.
  • Ultrasonic vocalization during social interaction and isolation in 2-week-old rats
    Hofer, M.A.; Shair, H.
  • Aversive effects of the synthetic cannabinoid CP 55,940 in rats
    McGregor, I.S.; Issakidis, C.N.; Prior, G.
  • Olfactory, thermal, and tactual influences on infantile ultrasonic vocalization in rats
    Oswalt, G.L.; Meier, G.W.

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