Biosynthesis and in vitro enzymatic synthesis of the isoleucine conjugate of 12-oxo-phytodienoic acid from the isoleucine conjugate of α-linolenic acid

Biosynthesis and in vitro enzymatic synthesis of the isoleucine conjugate of 12-oxo-phytodienoic... Bioorganic & Medicinal Chemistry Letters 28 (2018) 1020–1023 Contents lists available at ScienceDirect Bioorganic & Medicinal Chemistry Letters journal homepage: www.elsevier.com/locate/bmcl Biosynthesis and in vitro enzymatic synthesis of the isoleucine conjugate of 12-oxo-phytodienoic acid from the isoleucine conjugate of a-linolenic acid a a b a a Akira Uchiyama , Takaomi Yaguchi , Hiroyuki Nakagawa , Kento Sasaki , Naoshige Kuwata , a a,⇑ Hideyuki Matsuura , Kosaku Takahashi Division of Fundamental Agroscience Research, Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo 060-8589, Japan National Agriculture and Food Research Organization (NARO), Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan ar ti c l e i nf o ab stra ct Article history: The isoleucine conjugate of 12-oxo-phytodienoic acid (OPDA-Ile), a new member of the jasmonate family, Received 10 November 2017 was recently identified in Arabidopsis thaliana and might be a signaling molecule in plants. However, the Revised 11 February 2018 biosynthesis and function of OPDA-Ile remains elusive. This study reports an in vitro enzymatic method Accepted 14 February 2018 for synthesizing OPDA-Ile, which is catalyzed by reactions of lipoxygenase (LOX), allene oxide synthase Available online 15 February 2018 (AOS), and allene oxide cyclase (AOC) using http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Bioorganic & Medicinal Chemistry Letters Elsevier

Biosynthesis and in vitro enzymatic synthesis of the isoleucine conjugate of 12-oxo-phytodienoic acid from the isoleucine conjugate of α-linolenic acid

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier Ltd
ISSN
0960-894x
D.O.I.
10.1016/j.bmcl.2018.02.030
Publisher site
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Abstract

Bioorganic & Medicinal Chemistry Letters 28 (2018) 1020–1023 Contents lists available at ScienceDirect Bioorganic & Medicinal Chemistry Letters journal homepage: www.elsevier.com/locate/bmcl Biosynthesis and in vitro enzymatic synthesis of the isoleucine conjugate of 12-oxo-phytodienoic acid from the isoleucine conjugate of a-linolenic acid a a b a a Akira Uchiyama , Takaomi Yaguchi , Hiroyuki Nakagawa , Kento Sasaki , Naoshige Kuwata , a a,⇑ Hideyuki Matsuura , Kosaku Takahashi Division of Fundamental Agroscience Research, Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo 060-8589, Japan National Agriculture and Food Research Organization (NARO), Food Research Institute, 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan ar ti c l e i nf o ab stra ct Article history: The isoleucine conjugate of 12-oxo-phytodienoic acid (OPDA-Ile), a new member of the jasmonate family, Received 10 November 2017 was recently identified in Arabidopsis thaliana and might be a signaling molecule in plants. However, the Revised 11 February 2018 biosynthesis and function of OPDA-Ile remains elusive. This study reports an in vitro enzymatic method Accepted 14 February 2018 for synthesizing OPDA-Ile, which is catalyzed by reactions of lipoxygenase (LOX), allene oxide synthase Available online 15 February 2018 (AOS), and allene oxide cyclase (AOC) using

Journal

Bioorganic & Medicinal Chemistry LettersElsevier

Published: Apr 1, 2018

References

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