Journal of Virological Methods 247 (2017) 91–98 Contents lists available at ScienceDirect Journal of Virological Methods journal homepage: www.elsevier.com/locate/jviromet Biophysical characterization of inﬂuenza A virions MARK a a a a b Arun Parupudi , Flaviu Gruia , Samuel A. Korman , Sonia Dragulin-Otto , Kuldip Sra , a a, Richard L. Remmele Jr. , Jared S. Bee Analytical Sciences, MedImmune, One MedImmune Way, Gaithersburg, MD, United States Manufacturing Science and Technology, AstraZeneca, Liverpool, United Kingdom ARTICLE I NFO ABSTRACT Keywords: Antigenic drift of the inﬂuenza A virus requires that vaccine production is targeted to the strains circulating each Inﬂuenza vaccine virion year. Live-attenuated inﬂuenza A vaccine manufacturing is used to produce intact virions with the surface an- Particle counts tigens of the circulating strains. Inﬂuenza A typically contains a large percentage (> 90%) of non-infective Particle size virions. The ribonucleoprotein (RNP) content, virion structure, and aggregation are factors that are thought to Morphology have an impact on infectivity. However, these factors are diﬃcult to study because of the intrinsic variability in Biophysical characterization virion size, shape and overall structural integrity. Negative stain TEM for total particle counts and cryoTEM for detailed size/structural analysis are established benchmark techniques for virus
Journal of Virological Methods – Elsevier
Published: Sep 1, 2017
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