Biophysical characterization of influenza A virions

Biophysical characterization of influenza A virions Journal of Virological Methods 247 (2017) 91–98 Contents lists available at ScienceDirect Journal of Virological Methods journal homepage: www.elsevier.com/locate/jviromet Biophysical characterization of influenza A virions MARK a a a a b Arun Parupudi , Flaviu Gruia , Samuel A. Korman , Sonia Dragulin-Otto , Kuldip Sra , a a, Richard L. Remmele Jr. , Jared S. Bee Analytical Sciences, MedImmune, One MedImmune Way, Gaithersburg, MD, United States Manufacturing Science and Technology, AstraZeneca, Liverpool, United Kingdom ARTICLE I NFO ABSTRACT Keywords: Antigenic drift of the influenza A virus requires that vaccine production is targeted to the strains circulating each Influenza vaccine virion year. Live-attenuated influenza A vaccine manufacturing is used to produce intact virions with the surface an- Particle counts tigens of the circulating strains. Influenza A typically contains a large percentage (> 90%) of non-infective Particle size virions. The ribonucleoprotein (RNP) content, virion structure, and aggregation are factors that are thought to Morphology have an impact on infectivity. However, these factors are difficult to study because of the intrinsic variability in Biophysical characterization virion size, shape and overall structural integrity. Negative stain TEM for total particle counts and cryoTEM for detailed size/structural analysis are established benchmark techniques for virus http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2017.06.002
Publisher site
See Article on Publisher Site

Abstract

Journal of Virological Methods 247 (2017) 91–98 Contents lists available at ScienceDirect Journal of Virological Methods journal homepage: www.elsevier.com/locate/jviromet Biophysical characterization of influenza A virions MARK a a a a b Arun Parupudi , Flaviu Gruia , Samuel A. Korman , Sonia Dragulin-Otto , Kuldip Sra , a a, Richard L. Remmele Jr. , Jared S. Bee Analytical Sciences, MedImmune, One MedImmune Way, Gaithersburg, MD, United States Manufacturing Science and Technology, AstraZeneca, Liverpool, United Kingdom ARTICLE I NFO ABSTRACT Keywords: Antigenic drift of the influenza A virus requires that vaccine production is targeted to the strains circulating each Influenza vaccine virion year. Live-attenuated influenza A vaccine manufacturing is used to produce intact virions with the surface an- Particle counts tigens of the circulating strains. Influenza A typically contains a large percentage (> 90%) of non-infective Particle size virions. The ribonucleoprotein (RNP) content, virion structure, and aggregation are factors that are thought to Morphology have an impact on infectivity. However, these factors are difficult to study because of the intrinsic variability in Biophysical characterization virion size, shape and overall structural integrity. Negative stain TEM for total particle counts and cryoTEM for detailed size/structural analysis are established benchmark techniques for virus

Journal

Journal of Virological MethodsElsevier

Published: Sep 1, 2017

References

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