Biochemical analysis of NSs from different tospoviruses

Biochemical analysis of NSs from different tospoviruses •Binding of small and long dsRNA is likely generic to all Tospovirus NSs proteins.•dsRNA binding involves a NSs concentration-dependent two-step pattern.•NSs from Tsw breaking TSWV isolates may still bind dsRNA and suppress systemic RNA silencing.•This study stresses the importance of using purified proteins for biochemical assays. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Virus Research Elsevier

Biochemical analysis of NSs from different tospoviruses

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Publisher
Elsevier
Copyright
Copyright © 2017 The Authors
ISSN
0168-1702
D.O.I.
10.1016/j.virusres.2017.09.020
Publisher site
See Article on Publisher Site

Abstract

•Binding of small and long dsRNA is likely generic to all Tospovirus NSs proteins.•dsRNA binding involves a NSs concentration-dependent two-step pattern.•NSs from Tsw breaking TSWV isolates may still bind dsRNA and suppress systemic RNA silencing.•This study stresses the importance of using purified proteins for biochemical assays.

Journal

Virus ResearchElsevier

Published: Oct 15, 2017

References

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