Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events

Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of... Despite the impressive progress made in recent years in understanding the early steps in charge separation within the photosynthetic reaction centers, our knowledge of how ferredoxin (Fd) interacts with the acceptor side of photosystem I (PSI) is not as well developed. Fd accepts electrons after transiently docking to a binding site on the acceptor side of PSI. However, the exact location, as well as the stoichiometry, of this binding have been a matter of debate for more than two decades. Here, using Isothermal Titration Calorimetry (ITC) and purified components from wild type and mutant strains of the green algae Chlamydomonas reinhardtii we show that PSI has a single binding site for Fd, and that the association consists of two distinct binding events, each with a specific association constant. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochimica et Biophysica Acta (BBA) - Bioenergetics Elsevier

Binding of ferredoxin to algal photosystem I involves a single binding site and is composed of two thermodynamically distinct events

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
0005-2728
D.O.I.
10.1016/j.bbabio.2018.01.001
Publisher site
See Article on Publisher Site

Abstract

Despite the impressive progress made in recent years in understanding the early steps in charge separation within the photosynthetic reaction centers, our knowledge of how ferredoxin (Fd) interacts with the acceptor side of photosystem I (PSI) is not as well developed. Fd accepts electrons after transiently docking to a binding site on the acceptor side of PSI. However, the exact location, as well as the stoichiometry, of this binding have been a matter of debate for more than two decades. Here, using Isothermal Titration Calorimetry (ITC) and purified components from wild type and mutant strains of the green algae Chlamydomonas reinhardtii we show that PSI has a single binding site for Fd, and that the association consists of two distinct binding events, each with a specific association constant.

Journal

Biochimica et Biophysica Acta (BBA) - BioenergeticsElsevier

Published: Apr 1, 2018

References

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