Much about assembly processes dictating bio-cake microbiota remains uncertain, leading to poor understanding of membrane biofouling in membrane bioreactors (MBRs). This work aimed to reveal the underlying mechanisms driving bio-cake community during the biofouling process under different flux conditions. On the basis of 16S rRNA sequences, the results showed that bacterial diversity decreased with increasing fouling. Additionally, low-flux bio-cake (8 LMH) communities harbored much lower diversity than high-flux (16 LMH) bio-cake microbiomes. Ecological null model analyses and phylogenetic molecular ecological networks (pMENs) revealed that environmental filtering deterministically governed low-flux bio-cake communities. In contrast, high-flux bio-cake communities were mainly shaped in a stochastic manner. This is likely due to the higher stochastic deposition of bacterial taxa from bulk sludge because of the presence of a stronger drag force. Moreover, by lowering the flux, the interactions between bacterial lineages were enhanced; this is evidenced by the greater number of links, the higher average degree, and the higher average clustering coefficients within the pMENs in low-flux bio-cakes than those in high-flux bio-cakes. Most keystone fouling-related taxa in low-flux bio-cakes were motile and involved in nitrate reduction and polysaccharide/protein metabolism. This corroborated the important role of environmental filtering in the assembly process dictating low-flux bio-cake formation. Some low-abundance taxa were observed to be key fouling-related bacteria under both flux conditions, indicating that a few populations play paramount ecological roles in triggering biofouling. In summary, our findings clearly indicate distinct bio-cake community assembly patterns under different operational conditions and highlight the importance of developing specialized strategies for fouling control in individual MBR systems.
Water Research – Elsevier
Published: Jun 15, 2019
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera