Axonal damage and demyelination in the white matter after chronic cerebral hypoperfusion in the rat

Axonal damage and demyelination in the white matter after chronic cerebral hypoperfusion in the rat Cerebral white matter (WM) lesions are observed frequently in human ischemic cerebrovascular disease and have been thought to contribute to cognitive impairment. This type of lesion can be experimentally induced in rat brains under chronic cerebral hypoperfusion by the permanent occlusion of both common carotid arteries. However, it remains uncertain whether chronic ischemia can damage both the gray and white matter, and whether it can induce demyelination with or without axonal damage. Therefore, we examined axonal damage using immunohistochemistry for the amyloid β/A4 precursor protein (APP), chromogranin A (CgA) and demyelination using immunohistochemistry for the encephalitogenic peptide (EP) in this model. Severe WM lesions such as vacuolation and the loss of nerve fibers appeared in the optic nerve and optic tract after 3 days of ligation, and less intense changes were observed in the corpus callosum, internal capsule, and fiber bundles of the caudoputamen after 7 days with Klüver–Barrera and Bielschowsky staining. These WM lesions persisted even after 30 days. The APP, CgA, and EP-immunopositive fibers increased in number from 1 to 30 days after the ligation in the following WM regions: the optic nerve, optic tract, corpus callosum, internal capsule, and fiber bundles of the caudoputamen. In contrast, only a few APP, CgA, or EP-immunopositive fibers were detected in the gray matter regions, including the cerebral cortex and hippocampus. These results indicate that the WM is more susceptible to chronic cerebral hypoperfusion than the gray matter, with an involvement of both axonal and myelin components. Furthermore, immunohistochemistry for APP, CgA, and EP is far superior to routine histological staining in sensitivity and may become a useful tool to investigate WM lesions caused by various pathoetiologies. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Brain Research Elsevier

Axonal damage and demyelination in the white matter after chronic cerebral hypoperfusion in the rat

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Publisher
Elsevier
Copyright
Copyright © 2002 Elsevier Science B.V.
ISSN
0006-8993
D.O.I.
10.1016/S0006-8993(01)03223-1
Publisher site
See Article on Publisher Site

Abstract

Cerebral white matter (WM) lesions are observed frequently in human ischemic cerebrovascular disease and have been thought to contribute to cognitive impairment. This type of lesion can be experimentally induced in rat brains under chronic cerebral hypoperfusion by the permanent occlusion of both common carotid arteries. However, it remains uncertain whether chronic ischemia can damage both the gray and white matter, and whether it can induce demyelination with or without axonal damage. Therefore, we examined axonal damage using immunohistochemistry for the amyloid β/A4 precursor protein (APP), chromogranin A (CgA) and demyelination using immunohistochemistry for the encephalitogenic peptide (EP) in this model. Severe WM lesions such as vacuolation and the loss of nerve fibers appeared in the optic nerve and optic tract after 3 days of ligation, and less intense changes were observed in the corpus callosum, internal capsule, and fiber bundles of the caudoputamen after 7 days with Klüver–Barrera and Bielschowsky staining. These WM lesions persisted even after 30 days. The APP, CgA, and EP-immunopositive fibers increased in number from 1 to 30 days after the ligation in the following WM regions: the optic nerve, optic tract, corpus callosum, internal capsule, and fiber bundles of the caudoputamen. In contrast, only a few APP, CgA, or EP-immunopositive fibers were detected in the gray matter regions, including the cerebral cortex and hippocampus. These results indicate that the WM is more susceptible to chronic cerebral hypoperfusion than the gray matter, with an involvement of both axonal and myelin components. Furthermore, immunohistochemistry for APP, CgA, and EP is far superior to routine histological staining in sensitivity and may become a useful tool to investigate WM lesions caused by various pathoetiologies.

Journal

Brain ResearchElsevier

Published: Jan 4, 2002

References

  • Apoptosis in leukoaraiosis
    Brown, W.R.; Moody, D.M.; Thore, C.R.; Challa, V.R.
  • Diffuse white-matter disease in the geriatric population. A clinical, neuropathological, and CT study
    Goto, K.; Ishii, N.; Fukasawa, H.
  • Neurons induce the activation of microglial cells in vitro
    Sudo, S.; Tanaka, J.; Toku, K.; Desaki, J.; Matsuda, S.; Arai, T.; Sakanaka, M.; Maeda, N.
  • Experimental bilateral carotid artery occlusion: a study of the optic nerve in the rat
    Takamatsu, J.; Hirano, A.; Levy, D.; Henkind, P.
  • Cytotoxic effect of brain macrophages on developing neurons
    Thèry, C.; Chamak, B.; Mallat, M.
  • Cerebral blood flow and histopathological changes following permanent bilateral carotid artery ligation in Wistar rats
    Tsuchiya, M.; Sako, K.; Yura, S.; Yonemasu, Y.
  • Dose-dependent, protective effect of FK506 against white matter changes in the rat brain after chronic cerebral ischemia
    Wakita, H.; Tomimoto, H.; Akiguchi, I.; Kimura, J.
  • Amyloid precursor protein accumulates in white matter at the margin of a focal ischaemic lesion
    Yam, P.S.; Takasago, T.; Dewar, D.; Graham, D.I.; McCulloch, J.

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