Autoradiographic distribution of ( 3 H) l - N G -Nitro-arginine binding in rat brain

Autoradiographic distribution of ( 3 H) l - N G -Nitro-arginine binding in rat brain The distribution of nitric oxide synthase (NOS), the enzyme which produces nitric oxide, has previously been studied in the rat central nervous system (CNS) using the NADPH-diaphorase technique and anti-NOS antibodies. However, the former method may not always be selective for NOS while the latter is not quantitative. Therefore a selective, quantifiable method would be desirable. l - N G -Nitro-arginine, an inhibitor of NOS, is available in a tritiated form which we have shown to bind to NOS. We have now examined the regional distribution of ( 3 H) l - N G -nitro-arginine binding in the rat CNS using autoradiography. ( 3 H) l - N G -nitro-arginine specific binding was seen in a number of brain regions with the highest levels in the accessory olfactory bulb, the amygdaloid complex, the Islands of Calleja and the cerebellum. This regional distribution of ( 3 H) l - N G -nitro-arginine binding sites in the rat CNS was, in general, similar to that seen with the NADPH-diaphorase method and anti-NOS antibodies, consistent with the view that all three methods identify NOS in the CNS. Thus, ( 3 H) l - N G -nitro-arginine appears to be a useful radioligand for studying the distribution of NOS in the CNS as its binding is quantifiable and apparently selective for NOS. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neuropharmacology Elsevier

Autoradiographic distribution of ( 3 H) l - N G -Nitro-arginine binding in rat brain

Neuropharmacology, Volume 34 (1) – Jan 1, 1995

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Publisher
Elsevier
Copyright
Copyright © 1995 Elsevier Ltd
ISSN
0028-3908
eISSN
1873-7064
D.O.I.
10.1016/0028-3908(94)00132-C
Publisher site
See Article on Publisher Site

Abstract

The distribution of nitric oxide synthase (NOS), the enzyme which produces nitric oxide, has previously been studied in the rat central nervous system (CNS) using the NADPH-diaphorase technique and anti-NOS antibodies. However, the former method may not always be selective for NOS while the latter is not quantitative. Therefore a selective, quantifiable method would be desirable. l - N G -Nitro-arginine, an inhibitor of NOS, is available in a tritiated form which we have shown to bind to NOS. We have now examined the regional distribution of ( 3 H) l - N G -nitro-arginine binding in the rat CNS using autoradiography. ( 3 H) l - N G -nitro-arginine specific binding was seen in a number of brain regions with the highest levels in the accessory olfactory bulb, the amygdaloid complex, the Islands of Calleja and the cerebellum. This regional distribution of ( 3 H) l - N G -nitro-arginine binding sites in the rat CNS was, in general, similar to that seen with the NADPH-diaphorase method and anti-NOS antibodies, consistent with the view that all three methods identify NOS in the CNS. Thus, ( 3 H) l - N G -nitro-arginine appears to be a useful radioligand for studying the distribution of NOS in the CNS as its binding is quantifiable and apparently selective for NOS.

Journal

NeuropharmacologyElsevier

Published: Jan 1, 1995

References

  • Inhibition of rat cerebellar nitric oxide synthase by 7-nitro indazole and related substituted indazoles
    Babbedge, R.C.; Bland-Ward, P.A.; Hart, S.L.; Moore, P.K.
  • Nitric oxide: an ubiquitous messenger
    Berdeaux, A.
  • Evidence for nitric oxide synthase inhibitor-sensitive and insensitive hippocampal synaptic potentiation
    Gribkoff, V.K.; Lum-Ragan, J.T.
  • Demonstration and biochemical characterisation of rat brain NADPH-dependent diaphorase
    Kuonen, D.R.; Kemp, M.C.; Roberts, P.J.
  • Long-term depression of glutamate currents in cultured cerebellar Purkinje neurons does not require nitric oxide signalling
    Linden, D.J.; Connor, J.A.
  • Characterization of the binding of ( 3 H) l - N G -nitro-arginine in rat brain
    Michel, A.D.; Phul, R.K.; Stewart, T.L.; Humphrey, P.P.A.
  • L-N g -nitro-arginine (L-NOARG), a novel, L-arginine-reversible inhibitor of endothelium-dependent vasodilatation in vitro
    Moore, P.K.; al-Swayeh, O.A.; Chong, N.W.S.; Evans, R.A.; Gibson, A.
  • Characterization of the novel nitric oxide synthase inhibitor 7-nitro indazole and related indazoles: antinociceptive and cardiovascular effects
    Moore, P.K.; Wallace, P.; Gaffen, Z.; Hart, S.L.; Babbedge, R.C.

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