Arrangement of photosystem II supercomplexes in crystalline macrodomains within the thylakoid membrane of green plant chloroplasts 1 1 Edited by M.F. Moody

Arrangement of photosystem II supercomplexes in crystalline macrodomains within the thylakoid... The chloroplast thylakoid membrane of green plants is organized in stacked grana membranes and unstacked stroma membranes. We investigated the structural organization of Photosystem II (PSII) in paired grana membrane fragments by transmission electron microscopy. The membrane fragments were obtained by a short treatment of thylakoid membranes with the mild detergent n -dodecyl-α, d -maltoside and are thought to reflect the grana membranes in a native state. The membranes frequently show crystalline macrodomains in which PSII is organized in rows spaced by either 26.3 nm (large-spaced crystals) or 23 nm (small-spaced crystals). The small-spaced crystals are less common but better ordered. Image analysis of the crystals by an aperiodic approach revealed the precise positions of the core parts of PSII in the lattices, as well as features of the peripheral light-harvesting antenna. Together, they indicate that the so-called C 2 S 2 and C 2 S 2 M supercomplexes form the basic motifs of the small-spaced and large-spaced crystals, respectively. An analysis of a pair of membranes with a well-ordered large-spaced crystal reveals that many PSII complexes in one layer face only light-harvesting complexes (LHCII) in the other layer. The implications of this type of organization for the efficient transfer of excitation energy from LHCII to PSII and for the stacking of grana membranes are discussed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Molecular Biology Elsevier

Arrangement of photosystem II supercomplexes in crystalline macrodomains within the thylakoid membrane of green plant chloroplasts 1 1 Edited by M.F. Moody

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Publisher
Elsevier
Copyright
Copyright © 2000 Academic Press
ISSN
0022-2836
DOI
10.1006/jmbi.2000.4037
pmid
10966810
Publisher site
See Article on Publisher Site

Abstract

The chloroplast thylakoid membrane of green plants is organized in stacked grana membranes and unstacked stroma membranes. We investigated the structural organization of Photosystem II (PSII) in paired grana membrane fragments by transmission electron microscopy. The membrane fragments were obtained by a short treatment of thylakoid membranes with the mild detergent n -dodecyl-α, d -maltoside and are thought to reflect the grana membranes in a native state. The membranes frequently show crystalline macrodomains in which PSII is organized in rows spaced by either 26.3 nm (large-spaced crystals) or 23 nm (small-spaced crystals). The small-spaced crystals are less common but better ordered. Image analysis of the crystals by an aperiodic approach revealed the precise positions of the core parts of PSII in the lattices, as well as features of the peripheral light-harvesting antenna. Together, they indicate that the so-called C 2 S 2 and C 2 S 2 M supercomplexes form the basic motifs of the small-spaced and large-spaced crystals, respectively. An analysis of a pair of membranes with a well-ordered large-spaced crystal reveals that many PSII complexes in one layer face only light-harvesting complexes (LHCII) in the other layer. The implications of this type of organization for the efficient transfer of excitation energy from LHCII to PSII and for the stacking of grana membranes are discussed.

Journal

Journal of Molecular BiologyElsevier

Published: Sep 1, 2000

References

  • Structure and membrane organization of photosystem II in green plants
    Hankamer, B.; Barber, J.; Boekema, E.J.
  • Multiple crystal types reveal photosystem II to be a dimer
    Lyon, M.K.
  • Structure of the photosynthetic apparatus in protein-embedded chloroplasts
    Nicolson, G.L.
  • The correlation averaging of a regularly arranged bacterial cell envelope protein
    Saxton, O.; Baumeister, W.
  • Reversible particle movements associated with unstacking and restacking of chloroplast membranes in vitro
    Staehelin, L.A.
  • Energy transfer and trapping in photosynthesis
    Van Grondelle, R.; Dekker, J.P.; Gillbro, T.; Sundström, V.

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