Arachidonyl Ethanolamide (Anandamide) Activates the Parvocellular Part of Hypothalamic Paraventricular Nucleus

Arachidonyl Ethanolamide (Anandamide) Activates the Parvocellular Part of Hypothalamic... Arachidonyl ethanolamide, anandamide (ANA) was administered to male rats via a single i.p. injection at a dose of 0.02mg/kg. In an parallel experiment ANA injection was preceded by the injection of SR 141716 (1.0mg/kg), a selective and potent cannabinoid receptor antagonist. We observed using FOS protein immunocytochemistry that the parvocellular part of hypothalamic paraventricular nucleus (PVN) was activated as soon as 45 min. after ANA injection, i.e. the PVN showed an increased FOS immunoreactivity (FOSir). The peak level of FOSir was observed 90 min. after treatment. Meanwhile serum ACTH and corticosterone levels, as measured by radioimmunoassay, also significantly increased. 180 min. following drug injection both FOSir and serum hormone levels had returned to normal. SR 141716 did not antagonize these effects of ANA. We postulate that the locus of action of ANA the activation of the hypothalamo-pituitary-adrenal (HPA) axis is the parvocellular part of PVN. This activation may occur via a possible central cannabinoid receptor for which SR 141716 is not an effective antagonist. The rapid central response and activation of the HPA axis further support the view that ANA may be a central neurotransmitter or neuromodulator. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemical and Biophysical Research Communications Elsevier

Arachidonyl Ethanolamide (Anandamide) Activates the Parvocellular Part of Hypothalamic Paraventricular Nucleus

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Publisher
Elsevier
Copyright
Copyright © 1997 Academic Press
ISSN
0006-291x
DOI
10.1006/bbrc.1997.7222
pmid
9299434
Publisher site
See Article on Publisher Site

Abstract

Arachidonyl ethanolamide, anandamide (ANA) was administered to male rats via a single i.p. injection at a dose of 0.02mg/kg. In an parallel experiment ANA injection was preceded by the injection of SR 141716 (1.0mg/kg), a selective and potent cannabinoid receptor antagonist. We observed using FOS protein immunocytochemistry that the parvocellular part of hypothalamic paraventricular nucleus (PVN) was activated as soon as 45 min. after ANA injection, i.e. the PVN showed an increased FOS immunoreactivity (FOSir). The peak level of FOSir was observed 90 min. after treatment. Meanwhile serum ACTH and corticosterone levels, as measured by radioimmunoassay, also significantly increased. 180 min. following drug injection both FOSir and serum hormone levels had returned to normal. SR 141716 did not antagonize these effects of ANA. We postulate that the locus of action of ANA the activation of the hypothalamo-pituitary-adrenal (HPA) axis is the parvocellular part of PVN. This activation may occur via a possible central cannabinoid receptor for which SR 141716 is not an effective antagonist. The rapid central response and activation of the HPA axis further support the view that ANA may be a central neurotransmitter or neuromodulator.

Journal

Biochemical and Biophysical Research CommunicationsElsevier

Published: Aug 28, 1997

References

  • Biochem. Pharmacol.
    Shivachar, A.C.; Martin, B.R.; Ellis, E.F.

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