Application of yeast cells transformed with GFP expression constructs containing the RAD54 or RNR2 promoter as a test for the genotoxic potential of chemical substances

Application of yeast cells transformed with GFP expression constructs containing the RAD54 or... Yeast strains transformed with high copy number plasmids carrying the gene encoding a green fluorescent protein optimised for yeast (yEGFP3) under the control of the RAD54 or RNR2 promoter were used to investigate the activity of potentially DNA-damaging substances. The assays were performed on 96-well microtitre plates in the presence of different concentrations of the test substances. The synthesis of GFP protein was measured through the fluorescence signal and cell growth was monitored by absorption. Here, we demonstrate that this system can be used as a biosensor to assess the genotoxic potential of drugs and other chemical substances. The use of microtitre plates will enable full automation of the system and allows the inclusion of internal reference standards in each assay. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mutation Research - Genetic Toxicology and Environmental Mutagenesis Elsevier

Application of yeast cells transformed with GFP expression constructs containing the RAD54 or RNR2 promoter as a test for the genotoxic potential of chemical substances

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Publisher
Elsevier
Copyright
Copyright © 2000 Elsevier Science B.V.
ISSN
1383-5718
eISSN
1879-3592
D.O.I.
10.1016/S1383-5718(99)00209-0
Publisher site
See Article on Publisher Site

Abstract

Yeast strains transformed with high copy number plasmids carrying the gene encoding a green fluorescent protein optimised for yeast (yEGFP3) under the control of the RAD54 or RNR2 promoter were used to investigate the activity of potentially DNA-damaging substances. The assays were performed on 96-well microtitre plates in the presence of different concentrations of the test substances. The synthesis of GFP protein was measured through the fluorescence signal and cell growth was monitored by absorption. Here, we demonstrate that this system can be used as a biosensor to assess the genotoxic potential of drugs and other chemical substances. The use of microtitre plates will enable full automation of the system and allows the inclusion of internal reference standards in each assay.

Journal

Mutation Research - Genetic Toxicology and Environmental MutagenesisElsevier

Published: Jan 24, 2000

References

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