Application of PCR-RFLP analysis on species identification of canned tuna

Application of PCR-RFLP analysis on species identification of canned tuna The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was developed to identify the species of Thunnus thynnus , T . alalunga , T . obesus , T . albacares , Euthynnus pelamis ( Katsuwonus pelamis ), E . affinis , Auxis thazard and Sarda orientalis in products of canned tuna. To increase the efficiency and sensitivity of DNA extraction, a recent developed method of binding magnetic beads was applied in this study. Two sets of primer were designed to amplify 126 bp and 146 bp of partial mitochondrial cytochrome b gene, and five restriction enzymes including Bsp 1286I, Hin cII, Rsa I, Sca I and Mbo II were determined to analyze the short length fragments. The developed PCR-RFLP method was applied to authenticate species of 18 commercial canned tuna successfully, and it really provided a useful and academic technique to identify eight species in canned tuna. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Food Control Elsevier

Application of PCR-RFLP analysis on species identification of canned tuna

Food Control, Volume 18 (9) – Sep 1, 2007

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Publisher
Elsevier
Copyright
Copyright © 2006 Elsevier Ltd
ISSN
0956-7135
eISSN
1873-7129
D.O.I.
10.1016/j.foodcont.2006.07.001
Publisher site
See Article on Publisher Site

Abstract

The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was developed to identify the species of Thunnus thynnus , T . alalunga , T . obesus , T . albacares , Euthynnus pelamis ( Katsuwonus pelamis ), E . affinis , Auxis thazard and Sarda orientalis in products of canned tuna. To increase the efficiency and sensitivity of DNA extraction, a recent developed method of binding magnetic beads was applied in this study. Two sets of primer were designed to amplify 126 bp and 146 bp of partial mitochondrial cytochrome b gene, and five restriction enzymes including Bsp 1286I, Hin cII, Rsa I, Sca I and Mbo II were determined to analyze the short length fragments. The developed PCR-RFLP method was applied to authenticate species of 18 commercial canned tuna successfully, and it really provided a useful and academic technique to identify eight species in canned tuna.

Journal

Food ControlElsevier

Published: Sep 1, 2007

References

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