The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was developed to identify the species of Thunnus thynnus , T . alalunga , T . obesus , T . albacares , Euthynnus pelamis ( Katsuwonus pelamis ), E . affinis , Auxis thazard and Sarda orientalis in products of canned tuna. To increase the efficiency and sensitivity of DNA extraction, a recent developed method of binding magnetic beads was applied in this study. Two sets of primer were designed to amplify 126 bp and 146 bp of partial mitochondrial cytochrome b gene, and five restriction enzymes including Bsp 1286I, Hin cII, Rsa I, Sca I and Mbo II were determined to analyze the short length fragments. The developed PCR-RFLP method was applied to authenticate species of 18 commercial canned tuna successfully, and it really provided a useful and academic technique to identify eight species in canned tuna.
Food Control – Elsevier
Published: Sep 1, 2007
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