Application of modified Arxula adeninivorans yeast cells in an online biosensor for the detection of estrogenic compounds in wastewater samples

Application of modified Arxula adeninivorans yeast cells in an online biosensor for the detection... 1 Introduction</h5> The widespread use of natural and synthetic estrogens and chemicals with estrogenic activity is resulting in accumulation of estrogenic compounds in the environment. Already, at very low concentrations, these estrogenic molecules are affecting wildlife, particularly in the aquatic environment [1–4] . For this reason and also because of the potential effects on humans, practical analytical devices for detecting and quantifying estrogenic contamination are urgently needed. The majority of the analytical methods and bioassays on the market to date lack online continuous or semi-continuous operation. We have previously reported the development of the ‘EstraMonitor’ which is able to provide online semi-continuous analysis [5] . The EstraMonitor combines recombinant Arxula adeninivorans G1212/YRC102-hERα-phyK yeast cells as the microbial component with an amperometric detection method to analyze estrogenic contamination. A. adeninivorans G1212/YRC102-hERα-phyK was engineered to co-express the human estrogen receptor ( hERα ) gene and the inducible phytase ( phyK , derived from Klebsiella sp . ASR1) reporter gene under control of a promoter containing estrogen response elements (EREs) [6] . In the presence of estrogenic substances, such as 17β-estradiol (E2), the phyK gene is expressed and recombinant phytase is secreted into the media. The level of phytase is quantified http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Sensors and Actuators B: Chemical Elsevier

Application of modified Arxula adeninivorans yeast cells in an online biosensor for the detection of estrogenic compounds in wastewater samples

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Publisher
Elsevier
Copyright
Copyright © 2013 Elsevier B.V.
ISSN
0925-4005
eISSN
1873-3077
D.O.I.
10.1016/j.snb.2013.05.065
Publisher site
See Article on Publisher Site

Abstract

1 Introduction</h5> The widespread use of natural and synthetic estrogens and chemicals with estrogenic activity is resulting in accumulation of estrogenic compounds in the environment. Already, at very low concentrations, these estrogenic molecules are affecting wildlife, particularly in the aquatic environment [1–4] . For this reason and also because of the potential effects on humans, practical analytical devices for detecting and quantifying estrogenic contamination are urgently needed. The majority of the analytical methods and bioassays on the market to date lack online continuous or semi-continuous operation. We have previously reported the development of the ‘EstraMonitor’ which is able to provide online semi-continuous analysis [5] . The EstraMonitor combines recombinant Arxula adeninivorans G1212/YRC102-hERα-phyK yeast cells as the microbial component with an amperometric detection method to analyze estrogenic contamination. A. adeninivorans G1212/YRC102-hERα-phyK was engineered to co-express the human estrogen receptor ( hERα ) gene and the inducible phytase ( phyK , derived from Klebsiella sp . ASR1) reporter gene under control of a promoter containing estrogen response elements (EREs) [6] . In the presence of estrogenic substances, such as 17β-estradiol (E2), the phyK gene is expressed and recombinant phytase is secreted into the media. The level of phytase is quantified

Journal

Sensors and Actuators B: ChemicalElsevier

Published: Aug 1, 2013

References

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