The disruption of the normal expression of wnt -5a in cell lines and in tumors is becoming increasingly recognized as important in cell transformation and tumorigenesis. For example, in endometrial cancer wnt -5a is downregulated compared to normal tissue. Our laboratory has recently found that the ectopic expression of wnt -5a in human RCC23 renal carcinoma cells missing wnt -5a gene expression suppresses in vitro cell growth and telomerase enzyme activity. Furthermore, ectopic wnt -5a in MC-T16 uroepithelial cancer cells missing the region of chromosome 3p where wnt -5a has been mapped reverts uroepithelial cell tumorigenesis in athymic nude mice. These studies were based upon the previous finding that wnt -1 and wnt -2 transform C57MG mammary epithelial cells by downregulating the endogenous expression of wnt -5a. We now report that transfecting C57MG cells with a mammalian expression vector carrying antisense wnt -5a results in a cell phenotype that mimics cell transformation by ectopic wnt -1 or wnt -2. Correspondingly, wnt -1-transformed cells are partially reverted in the presence of ectopic wnt -5a. We conclude from this that wnt -5a is an important regulator of cell growth and differentiation and its loss of expression leads to cell transformation.
Experimental Cell Research – Elsevier
Published: May 25, 1998
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera