Anti-hypertensive activity of genetically modified soybean seeds accumulating novokinin

Anti-hypertensive activity of genetically modified soybean seeds accumulating novokinin Novokinin (Arg-Pro-Leu-Lys-Pro-Trp), which has been designed based on the structure of ovokinin (2–7), significantly reduces the systolic blood pressure at a dose of 100 μg/kg after oral administration in spontaneously hypertensive rats (SHRs). In this study, we generated a transgenic soybean which accumulates novokinin. A vector encoding a modified β-conglycinin α′ subunit (4novokinin-α′) in which four novokinin sequences have been incorporated by site-directed mutagenesis was introduced into somatic embryos by whisker-mediated gene transformation to produce a transgenic soybean. The 4novokinin-α′ occupied 0.5% of total soluble protein and 5% of the β-conglycinin α′ subunit in the transgenic soybean seeds. Protein extracted from the transgenic soybean reduced systolic blood pressure after single oral administration in SHRs at a dose of 0.15 g/kg. Defatted flour from the transgenic soybean also reduced the systolic blood pressure at a dose of 0.25 g/kg. Thus, the 4novokinin-α′ produced in soybean exhibited an anti-hypertensive activity in SHRs after oral administration. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Peptides Elsevier

Anti-hypertensive activity of genetically modified soybean seeds accumulating novokinin

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Publisher
Elsevier
Copyright
Copyright © 2007 Elsevier Inc.
ISSN
0196-9781
D.O.I.
10.1016/j.peptides.2007.11.018
Publisher site
See Article on Publisher Site

Abstract

Novokinin (Arg-Pro-Leu-Lys-Pro-Trp), which has been designed based on the structure of ovokinin (2–7), significantly reduces the systolic blood pressure at a dose of 100 μg/kg after oral administration in spontaneously hypertensive rats (SHRs). In this study, we generated a transgenic soybean which accumulates novokinin. A vector encoding a modified β-conglycinin α′ subunit (4novokinin-α′) in which four novokinin sequences have been incorporated by site-directed mutagenesis was introduced into somatic embryos by whisker-mediated gene transformation to produce a transgenic soybean. The 4novokinin-α′ occupied 0.5% of total soluble protein and 5% of the β-conglycinin α′ subunit in the transgenic soybean seeds. Protein extracted from the transgenic soybean reduced systolic blood pressure after single oral administration in SHRs at a dose of 0.15 g/kg. Defatted flour from the transgenic soybean also reduced the systolic blood pressure at a dose of 0.25 g/kg. Thus, the 4novokinin-α′ produced in soybean exhibited an anti-hypertensive activity in SHRs after oral administration.

Journal

PeptidesElsevier

Published: Mar 1, 2008

References

  • Engineered GFP as a vital reporter in plants
    Chiu, W.; Niwa, Y.; Zeng, W.; Hirano, T.; Kobayashi, H.; Sheen, J.
  • High-level expression of basic fibroblast growth factor in transgenic soybean seeds and characterization of its biological activity
    Ding, S.H.; Huang, L.Y.; Wang, Y.D.; Sun, H.C.; Xiang, Z.H.
  • One-step purification of Kunitz soybean trypsin inhibitor
    Duranti, M.; Barbiroli, A.; Scarafoni, A.; Tedeschi, G.; Morazzoni, P.
  • Accumulation of soybean glycinin and its assembly with the glutelins in rice
    Katsube, T.; Kurisaka, N.; Ogawa, M.; Maruyama, N.; Ohtsuka, R.; Utsumi, S.; Takaiwa, F.
  • Inheritance of alleles for Cgy 1 and Gy 4 storage protein genes in soybean
    Kitamura, K.; Davies, C.S.; Nielsen, N.C.
  • Hypotensive activity of novokinin, a potent analogue of ovokinin(2–7), is mediated by angiotensin AT 2 receptor and prostaglandin IP receptor
    Yamada, Y.; Yamauchi, D.; Usui, H.; Zhao, H.; Yokoo, M.; Ohinata, K.; Iwai, M.; Horiuchi, M.; Yoshikawa, M.

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