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Search ADS PubMed WorldCat 15. Duncan MW , Lidgard RO . Quantitative analysis of low molecular weight biomolecules by MALDI - TOF . 43rd American Society of Mass Spectrometry , Vol . Atlanta GA . 1995 . 16 ...
-buffered saline HP healthy plasma HS healthy serum ESI- TOF -MS electrospray ionization time-of-flight mass spectrometry FWHM full width at half maximum PCA principal component analysis AUC area under ...
) acetonitrile, 0.45% (vol/vol) trifluoroacetic acid (TFA)] into the pipette and depositing onto a MALDI target. MASS SPECTROMETRY We performed MALDI - TOF mass spectrometry (MS) by use of a Bruker Ultraflex III ...
, considered the crude sphingolipid fraction, was stored at − 70°C for MALDI - TOF MS analysis . DE MALDI - TOF MS analysis Each sample obtained from the above procedure was dissolved in 5 μL chloroform–methanol (v/v ...
performed using conventional approaches (28)(29) but is also possible using mass spectrometry by switching to a biased analysis that automatically assumes GcG is present and then asks a further question ...
with electrospray-tandem mass spectrometry analysis on the IEF-separated fractions of the BQ-adducted cytochrome c. The charges were assigned using extensible systematic force field parameters (Discover 3.0 ESFF ...
for average molecular mass determination Glycation of both α and β hemoglobin subunits was analysed by matrix-assisted desorption/ionization time-of-flight mass spectrometry ( MALDI - TOF MS) for mass shift ...
desorption ionization ( MALDI ) mass spectrometry . The mass spectra of the purified peptide were recorded on Autoflex III TOF / TOF MS ( MALDI - TOF mass spectrometer, Bruker Daltonics Co.). Briefly, 2 μl of 5 μM ...
with significant clinical value [16, 17]. As an analytical technique for the quantification of disease-associated alterations, two-dimensional electrophoresis (2-DE) combined with mass spectrometry is often applied ...
showing allele-specific binding to the C allele was identified as the alpha subunit of GABP using MALDI TOF / TOF mass spectrometry . In EMSA supershift assays using antibodies to GABP-α, we observed ...
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