An increased expression of nucleolin is associated with a physiological nucleolar segregation

An increased expression of nucleolin is associated with a physiological nucleolar segregation Nucleolar segregation is the most striking cellular phenotypic feature of cold-acclimatized carp and depicts the cyclical reprogramming that the physiology of the fish undergoes between summer and winter, where a clear differential expression of some nucleolar related genes occurs. We characterized carp nucleolin, a nucleolar protein involved in multiple steps of ribosome biogenesis, and evaluated its expression upon fish acclimatization. We show that the carp cDNA deduced amino acid sequence exhibits the same tripartite structural organization found in other species. Nevertheless, we observed that nucleolin mRNA expression was strongly induced in the cold-adapted carp as was the nuclear protein content, assessed by immunocytochemistry in liver sections. The physiological up-regulation of nucleolin in the cold-acclimatized carp, where rRNA transcription and processing are depressed concomitantly with the nucleolus segregation, is consistent with the notion that nucleolin plays a fundamental role in repressing rRNA synthesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemical and Biophysical Research Communications Elsevier

An increased expression of nucleolin is associated with a physiological nucleolar segregation

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Publisher
Elsevier
Copyright
Copyright © 2002 Elsevier Science (USA)
ISSN
0006-291x
D.O.I.
10.1016/S0006-291X(02)02978-9
Publisher site
See Article on Publisher Site

Abstract

Nucleolar segregation is the most striking cellular phenotypic feature of cold-acclimatized carp and depicts the cyclical reprogramming that the physiology of the fish undergoes between summer and winter, where a clear differential expression of some nucleolar related genes occurs. We characterized carp nucleolin, a nucleolar protein involved in multiple steps of ribosome biogenesis, and evaluated its expression upon fish acclimatization. We show that the carp cDNA deduced amino acid sequence exhibits the same tripartite structural organization found in other species. Nevertheless, we observed that nucleolin mRNA expression was strongly induced in the cold-adapted carp as was the nuclear protein content, assessed by immunocytochemistry in liver sections. The physiological up-regulation of nucleolin in the cold-acclimatized carp, where rRNA transcription and processing are depressed concomitantly with the nucleolus segregation, is consistent with the notion that nucleolin plays a fundamental role in repressing rRNA synthesis.

Journal

Biochemical and Biophysical Research CommunicationsElsevier

Published: Jan 31, 2003

References

  • Transcription factor pit-1 expression is modulated upon seasonal acclimatization of eurythermal ectotherms: identification of two pit-1 genes in the carp
    Kausel, G.; Vera, M.I.; San Martin, R.; Figueroa, J.; Molina, A.; Muller, M.; Martial, J.; Krauskopf, M.
  • Gene structure of the carp fish ribosomal protein L41: seasonally regulated expression
    Molina, A.; Corta, A.; San Martin, R.; Alvarez, M.; Burzio, L.O.; Krauskopf, M.; Vera, M.I.
  • Functional and dynamic aspects of the mammalian nucleolus
    Scheer, U.; Benavente, R.
  • Nucleolin is a sequence-specific RNA-binding protein: characterization of targets on pre-ribosomal RNA
    Ghisolfi-Nieto, L.; Joseph, G.; Puvion-Dutilleul, F.; Amalric, F.; Bouvet, P.
  • Nucleolin functions in the first step of ribosomal RNA processing
    Ginisty, H.; Amalric, F.; Bouvet, P.

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