An in vitro rabbit retina model to study electrophysiologic and metabolic function during and following ischemia

An in vitro rabbit retina model to study electrophysiologic and metabolic function during and... Most in vitro studies involving neuronal ischemia use biochemical measures and/or cell counting to assess cellular death. We describe an in vitro rabbit retina model in which we measured glucose utilization, lactate production, and light-evoked compound action potentials (CAPs) to assess metabolic and functional recovery following ischemia. Under control conditions, retinal glucose utilization and lactate production ( n =7), as well as CAPs ( n =8) remained quite constant for 6–8 h. During ischemia (glucose reduced from 6 to 1 mM and oxygen from 95 to 15%), glucose utilization and lactate production fell to 50%. CAPs fell to 50% in 3–4 min, and to 0% in 8–10 min. Recovery during 3–4 h of ‘return-to-control’ was dependent upon the length of ischemia. Glucose utilization recovered to 63% after 1 h ( n =4) and to 18% after 2 h of ischemia ( n =6, P <0.001). Lactate production recovered to 77% after 1 h ( n =4) and to 54% after 2 h of ischemia ( n =6, P <0.001). CAPs returned to 51, 15, and 0.13% of the control responses after 0.5 h ( n =7), 1 h ( n =8), and 2 h ( n =5) of ischemia, respectively ( P <0.001). This avascular, blood–brain barrier-free preparation provides an opportunity to use both metabolic and functional criteria to test protection against neuronal ischemia. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Neuroscience Methods Elsevier

An in vitro rabbit retina model to study electrophysiologic and metabolic function during and following ischemia

Loading next page...
 
/lp/elsevier/an-in-vitro-rabbit-retina-model-to-study-electrophysiologic-and-zRQqhp2IO6
Publisher
Elsevier
Copyright
Copyright © 1999 Elsevier Science B.V.
ISSN
0165-0270
eISSN
1872-678X
D.O.I.
10.1016/S0165-0270(99)00068-0
Publisher site
See Article on Publisher Site

Abstract

Most in vitro studies involving neuronal ischemia use biochemical measures and/or cell counting to assess cellular death. We describe an in vitro rabbit retina model in which we measured glucose utilization, lactate production, and light-evoked compound action potentials (CAPs) to assess metabolic and functional recovery following ischemia. Under control conditions, retinal glucose utilization and lactate production ( n =7), as well as CAPs ( n =8) remained quite constant for 6–8 h. During ischemia (glucose reduced from 6 to 1 mM and oxygen from 95 to 15%), glucose utilization and lactate production fell to 50%. CAPs fell to 50% in 3–4 min, and to 0% in 8–10 min. Recovery during 3–4 h of ‘return-to-control’ was dependent upon the length of ischemia. Glucose utilization recovered to 63% after 1 h ( n =4) and to 18% after 2 h of ischemia ( n =6, P <0.001). Lactate production recovered to 77% after 1 h ( n =4) and to 54% after 2 h of ischemia ( n =6, P <0.001). CAPs returned to 51, 15, and 0.13% of the control responses after 0.5 h ( n =7), 1 h ( n =8), and 2 h ( n =5) of ischemia, respectively ( P <0.001). This avascular, blood–brain barrier-free preparation provides an opportunity to use both metabolic and functional criteria to test protection against neuronal ischemia.

Journal

Journal of Neuroscience MethodsElsevier

Published: Aug 15, 1999

References

  • The rabbit retina in vitro
    Ames, A.; Masland, R.H.
  • In vitro retina as an experimental model of the central nervous system
    Ames, A.; Nesbett, F.B.
  • Acetylsalicyclate administered during simulated ischemia reduces the recovery of neuronal function in the in vitro rabbit retina
    Maynard, K.I.; Arango, P.M.; Chen, D.; Ogilvy, C.S.
  • In vivo and in vitro experiments show that betaxolol is a retinal neuroprotective agent
    Osborne, N.N.; Cazevieille, C.; Carvalho, A.L.; Larsen, A.K.; DeSantis, L.
  • Protein synthesis in central nervous tissue: studies on retina in vitro
    Parks, J.M.; Ames, A.; Nesbett, F.B.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off