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of the biomarker of interest; its optimization is based also on in-silico determination of the aptamer nucleotides interactions with the protein of interest. We demonstrate this scheme for the case of Prostate ...
retaining their native conformations. The cell surface is complex and has many different molecules, especially proteins. Cell-SELEX can produce aptamers that specifically bind to a certain cell line based ...
of recurrence [4]. Serum tumor markers, such as prostate - specific antigen (PSA), alpha-fetoprotein, cancer antigen 15-3 (CA 15-3), cancer antigen 125 (CA 125), cancer antigen 19-9 (CA 19-9), cancer antigen 27.29 ...
with these premises: both subtypes express prostate specific antigen (PSA), prostatic acid phosphatase (PAP), p53 antigen and Rb protein (ATCC.org). Differential levels of proteins in VCaP versus LNCaP cell exosomes ...
at an acceleration voltage of 200 kV. Fluorescence resonance energy transfer (FRET) decrease assay The AF488-labeled TDNs –Evs (the aptamers of TDNs were labeled by AF488) and AF488-labeled TDNs were incubated with 20 ...
, cantilevers, and other nanotechnologies in promising diagnostic applications. Results: Several nanodiagnostic assays have been developed, including a QD- based assay capable of detecting biotinylated prostate ...
light scattering analysis. Indeed, not-annealed V7t1 forms both monomeric and dimeric G-quadruplexes, while the annealed oligonucleotide is a monomeric species. Remarkably, only the dimeric aptamer ...
, and facile surface modification.21 Gold NPs can strongly enhance optical processes such as light absorption, scattering , fluorescence, and surface-enhanced Raman scattering (SERS) due to the unique interaction ...
assay is a fast and reproducible method of assessing genomic instability through the measurement of fluorescently labeled antibodies to specific membrane proteins by flow-cytometric methodology. We ...
of the tumor cells because they will shed light on the mutations or the over- and under-regulation of specific tumor-related genes. Among the molecular techniques used for mutational profiling are Sanger ...
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