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Abstract A new approach for the synthesis of randomized DNA sequences containing the 20 codons corresponding to all natural amino acids is described. The strategy is based on the use of dinucleotide ...
). Library III was also assembledwith the 50 mM mixture of Fmoc-phosphoramidites, but in this case with the on-line mixing protocol. Assembly of the libraries required the use of two DNA synthesizers, one ...
characteristics, we have now devised TrimerDimer, a novel codon-based saturation mutagenesis approach that removes redundant and stop codons during the assembly of degenerate oligonucleotides. In this approach ...
for creating targeted mutations in E. chaffeensis by employing both homologous recombination and TargeTron methods, and random mariner mutagenesis using the Himar1 transposase system. Six different genomic ...
-prone PCR (epPCR; Zakour and Loeb, 1982; Leung et al., 1989; Cadwell and Joyce, 1992) and DNA shuffling (Crameri et al., 1998), are used in vitro to target the mutagenesis to specific DNA sequences ...
-site mutagenesis methods because the success in both cases is determined by the necessity to acquire a single clone with desired alterations. Conversely, the success of randomization approaches ...
Abstract We have used random sequence mutagenesis to generate mutants of DNA polymerase β in an effort to identify amino acid residues important for function, catalytic efficiency and fidelity ...
the usefulness of random mutagenesis for the studies on the hyperthermophile, as well as crucial roles of tRNA modifications in cellular thermotolerance. INTRODUCTION The hyperthermotolerance/hyperthermophily ...
Introduction The combination of transposon mutagenesis with next-generation sequencing revolutionized the study of gene essentiality [1–4]. This approach is based on the random integration ...
uniform distribution of insertions. Ideally, for practical use as a tool for mutagenesis and DNA sequencing, an in vitro transposition system must readily yield large numbers of recoverable, independent ...
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