An amperometric biosensor based on multiwalled carbon nanotube-poly(pyrrole)-horseradish peroxidase nanobiocomposite film for determination of phenol derivatives

An amperometric biosensor based on multiwalled carbon nanotube-poly(pyrrole)-horseradish... An amperometric biosensor based on horseradish peroxidase (HRP) and carbon nanotube (CNT)/polypyrrole (PPy) nanobiocomposite film on a gold surface has been developed. The HRP was incorporated into the CNT/PPy nanocomposite matrix in one-step electropolymerization process without the aid of cross-linking agent. Amperometric response was measured as a function of concentration of phenol derivatives, at a fixed bias voltage of −50 mV. Optimization of the experimental parameters was performed with regard to pH and concentration of hydrogen peroxide. The linear range, sensitivity and detection limit of the biosensor were investigated for eighteen phenol derivatives. The sensitivity in the linear range increased in this order: 4-methoxyphenol > 2-aminophenol > guaiacol = m -cresol > 2-chlorophenol = 4-chlorophenol = hydroquinone = pyrocatechol > 2,6-dimethoxyphenol > 3-chlorophenol > p- cresol > p -benzoquinone = 4-acetamidophenol > catechol > phenol = pyrogallol = 2,4-dimethylphenol. CNTs was shown to enhance the electron transfer as a mediator and capable to carry higher bioactivity owing to its intensified surface area. The biosensor exhibited low detection limits with a short response time (2 s) for the tested phenolics compared to the reported working electrodes. It retained 70% of its initial activity after using for 700 measurements in 1 month. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Talanta Elsevier

An amperometric biosensor based on multiwalled carbon nanotube-poly(pyrrole)-horseradish peroxidase nanobiocomposite film for determination of phenol derivatives

Talanta, Volume 76 (5) – Sep 15, 2008

Loading next page...
 
/lp/elsevier/an-amperometric-biosensor-based-on-multiwalled-carbon-nanotube-poly-0Ag585y3pF
Publisher
Elsevier
Copyright
Copyright © 2008 Elsevier B.V.
ISSN
0039-9140
eISSN
1873-3573
DOI
10.1016/j.talanta.2008.05.016
Publisher site
See Article on Publisher Site

Abstract

An amperometric biosensor based on horseradish peroxidase (HRP) and carbon nanotube (CNT)/polypyrrole (PPy) nanobiocomposite film on a gold surface has been developed. The HRP was incorporated into the CNT/PPy nanocomposite matrix in one-step electropolymerization process without the aid of cross-linking agent. Amperometric response was measured as a function of concentration of phenol derivatives, at a fixed bias voltage of −50 mV. Optimization of the experimental parameters was performed with regard to pH and concentration of hydrogen peroxide. The linear range, sensitivity and detection limit of the biosensor were investigated for eighteen phenol derivatives. The sensitivity in the linear range increased in this order: 4-methoxyphenol > 2-aminophenol > guaiacol = m -cresol > 2-chlorophenol = 4-chlorophenol = hydroquinone = pyrocatechol > 2,6-dimethoxyphenol > 3-chlorophenol > p- cresol > p -benzoquinone = 4-acetamidophenol > catechol > phenol = pyrogallol = 2,4-dimethylphenol. CNTs was shown to enhance the electron transfer as a mediator and capable to carry higher bioactivity owing to its intensified surface area. The biosensor exhibited low detection limits with a short response time (2 s) for the tested phenolics compared to the reported working electrodes. It retained 70% of its initial activity after using for 700 measurements in 1 month.

Journal

TalantaElsevier

Published: Sep 15, 2008

References

  • Sens. Actuators B: Chem.
    Yang, S.; Li, Y.; Jiang, X.; Chen, Z.; Lin, X.
  • Biosens. Bioelectron.
    Sotiropoulou, S.; Gavalas, V.; Vamvakaki, V.; Chaniotakis, N.A.
  • Biosens. Bioelectron.
    Chang, S.C.; Rawson, K.; McNeil, C.J.
  • Biosens. Bioelectron.
    Fan, Q.; Shan, D.; Xue, H.; He, Y.; Cosnier, S.
  • Analyst
    Gorton, L.; Jönsson-Petersson, G.; Csöregi, E.; Johansson, K.; Dominguez, E.; Marko-Varga, G.

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off