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and robust Agrobacterium - mediated transient expression system , named AGROBEST ( Agrobacterium - mediated enhanced seedling transformation), which achieves versatile analysis of diverse gene functions in intact ...
. An Agrobacterium ‐ mediated transient gene expression system for intact leaves. Plant Science 122, 101–108. Google Scholar Luehrsen KR, de Wet JR, Walbot V. 1992. Transient expression analysis in plants using firefly ...
the efficiency and full functionality of vector constructs is highly desirable.ResultsHere, we report an Agrobacterium - mediated transient transformation assay with intact sorghum leaves using green fluorescent ...
Delivery of small interfering RNA (siRNA) to intact plants for gene silencing mainly relies on viral vectors and Agrobacterium ‐ mediated transformation due to the barrier of intact plant cell wall ...
Delivery of small interfering RNA (siRNA) to intact plants for gene silencing mainly relies on viral vectors and Agrobacterium ‐ mediated transformation due to the barrier of intact plant cell wall ...
insusceptibility of monocots to Agrobacterium - mediated transformation. We have circumvented these difficulties by transferring foreign genes directly into the intact cells (with cell wails) of three important crop ...
A convenient, Agrobacterium ‐ mediated transient expression assay has been evaluated for rapid analysis of plant promoters and transcription factors in vivo. By simple infiltration of Agrobacterium ...
-infected with Agrobacterium -carrying iAc construct for transient expression of transposase to generate stable Ds-tagged mutants in the T0 generation. All nine regenerants were PCR-positive for Ds. However ...
various plant gene expression technologies currently available, transient gene overexpression in Nicotiana benthamiana based on Agrobacterium tumefaciens ‐ mediated delivery of viral or non‐viral vectors ...
Abstract The Agrobacterium - mediated transient expression assay in intact tissues has emerged as a rapid and useful method to analyze genes and gene products in plants. In many cases, high levels ...
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