Bioresource Technology 249 (2018) 417–424 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech Amino acids in cell wall of Gram-positive bacterium Micrococcus sp. hsn08 with ﬂocculation activity on Chlorella vulgaris biomass a a b a, Yi Li , Yanting Xu , Tianling Zheng , Hailei Wang College of Life Sciences, Henan Normal University, Xinxiang 453007, China State Key Laboratory of Marine Environmental Science, School of Life Sciences, Xiamen University, Xiamen 361005, China GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: The aim of this work was to investigate the ﬂocculation mechanism by Gram-positive bacterium, Micrococcus sp. Micrococcus sp. hsn08 hsn08 as a means for harvesting Chlorella vulgaris biomass. Bacterial cells of Micrococcus sp. hsn08 were added Chlorella vulgaris into algal culture to harvest algal cells through direct contacting with algae to form ﬂocs. Viability dependence Amino acids in cell wall test conﬁrmed that ﬂocculation activity does not depend on live bacteria, but on part of the peptidoglycan. The Ions bridging further investigation has determined that amino acids in cell wall play an important role to ﬂocculate algal cells. Flocculation mechanism Positively charged calcium can combine bacterial and algal cells together, and form a bridge between them,
Bioresource Technology – Elsevier
Published: Feb 1, 2018
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