Aging alters macrophage properties in human skeletal muscle both at rest and in response to acute resistance exercise

Aging alters macrophage properties in human skeletal muscle both at rest and in response to acute... Macrophages are involved in skeletal muscle repair through pro-inflammatory and alternative functions. We tested the hypothesis that aging alters the abundance and properties of skeletal muscle macrophages that will influence their functional response to acute resistance exercise. Total macrophages (CD68+), as well as pro- (CD11b+) and anti-inflammatory (CD163+) subpopulations and associated cytokine mRNAs were quantified in vastus lateralis biopsies from young ( N =17) and elderly ( N =17) males pre- and 72 h post-exercise. Pre-exercise, young muscle tended to possess a greater number of macrophages, whereas elderly muscle possessed higher levels of IL-1β ( P =0.001), IL-1RA ( P =0.003), and IL-10 ( P =0.028). Post-exercise, total macrophages did not change in either group, however, the number of CD11b+ ( P =0.039) and CD163+ ( P =0.026) cells increased 55 and 29%, respectively, but only in the young. IL-1β ( P =0.006), IL-10 ( P =0.016), and AMAC-1 ( P =0.044) also increased, approximately two-fold, and again only in the young. Quantitation of CD11b+ and CD163+ cells suggests that the majority of resident macrophages possess alternative functions, and a small subpopulation participates in the inflammatory response. Both subpopulations increased their activity post-exercise, exclusively in the young. These findings suggest that aging results in a defective regulation of muscle macrophage function, both at baseline and in response to resistance exercise, that may limit muscle hypertrophy in older adults. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Experimental Gerontology Elsevier

Aging alters macrophage properties in human skeletal muscle both at rest and in response to acute resistance exercise

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Publisher
Elsevier
Copyright
Copyright © 2006 Elsevier Inc.
ISSN
0531-5565
eISSN
1873-6815
D.O.I.
10.1016/j.exger.2005.12.007
Publisher site
See Article on Publisher Site

Abstract

Macrophages are involved in skeletal muscle repair through pro-inflammatory and alternative functions. We tested the hypothesis that aging alters the abundance and properties of skeletal muscle macrophages that will influence their functional response to acute resistance exercise. Total macrophages (CD68+), as well as pro- (CD11b+) and anti-inflammatory (CD163+) subpopulations and associated cytokine mRNAs were quantified in vastus lateralis biopsies from young ( N =17) and elderly ( N =17) males pre- and 72 h post-exercise. Pre-exercise, young muscle tended to possess a greater number of macrophages, whereas elderly muscle possessed higher levels of IL-1β ( P =0.001), IL-1RA ( P =0.003), and IL-10 ( P =0.028). Post-exercise, total macrophages did not change in either group, however, the number of CD11b+ ( P =0.039) and CD163+ ( P =0.026) cells increased 55 and 29%, respectively, but only in the young. IL-1β ( P =0.006), IL-10 ( P =0.016), and AMAC-1 ( P =0.044) also increased, approximately two-fold, and again only in the young. Quantitation of CD11b+ and CD163+ cells suggests that the majority of resident macrophages possess alternative functions, and a small subpopulation participates in the inflammatory response. Both subpopulations increased their activity post-exercise, exclusively in the young. These findings suggest that aging results in a defective regulation of muscle macrophage function, both at baseline and in response to resistance exercise, that may limit muscle hypertrophy in older adults.

Journal

Experimental GerontologyElsevier

Published: Mar 1, 2006

References

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