Activation of LEDGF Gene by Thermal- and Oxidative-Stresses

Activation of LEDGF Gene by Thermal- and Oxidative-Stresses LEDGF promotes survival of many cell types against a wide range of environmental stresses, and cells responding to those stresses expressed higher levels of heat shock proteins (Hsps). LEDGF/p75 is a weak coactivator of general transcription. We speculated that a stress signal may activate expression of the LEDGF gene, resulting in elevated levels of LEDGF which may activate expression of stress-related proteins to protect cells from stresses. Lens epithelial cells (LECs) and cos7 cells were cultured under heat- or oxidative-stress. After cells were cultured for defined time, we quantified LEDGF mRNA and LEDGF protein with RT-PCR, Northern blot, and protein blot analysis. Our results showed that higher levels of LEDGF were found in both cell types under heat- and oxidative-stress than that in cells cultured at nonstress condition. Thus, one of the primary events in the stress-related protective events is the activation of LEDGF, a regulatory protein. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biochemical and Biophysical Research Communications Elsevier

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Publisher
Elsevier
Copyright
Copyright © 2000 Academic Press
ISSN
0006-291x
D.O.I.
10.1006/bbrc.2000.3606
Publisher site
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Abstract

LEDGF promotes survival of many cell types against a wide range of environmental stresses, and cells responding to those stresses expressed higher levels of heat shock proteins (Hsps). LEDGF/p75 is a weak coactivator of general transcription. We speculated that a stress signal may activate expression of the LEDGF gene, resulting in elevated levels of LEDGF which may activate expression of stress-related proteins to protect cells from stresses. Lens epithelial cells (LECs) and cos7 cells were cultured under heat- or oxidative-stress. After cells were cultured for defined time, we quantified LEDGF mRNA and LEDGF protein with RT-PCR, Northern blot, and protein blot analysis. Our results showed that higher levels of LEDGF were found in both cell types under heat- and oxidative-stress than that in cells cultured at nonstress condition. Thus, one of the primary events in the stress-related protective events is the activation of LEDGF, a regulatory protein.

Journal

Biochemical and Biophysical Research CommunicationsElsevier

Published: Oct 5, 2000

References

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