A YAC contig containing the reeler locus with preliminary characterization of candidate gene fragments

A YAC contig containing the reeler locus with preliminary characterization of candidate gene... The reeler mutation in the mouse maps to proximal chromosome 5 and defines a key gene involved in brain development and evolution. No gene product is known, and the locus is currently being characterized by positional cloning. YAC clones corresponding to the closest markers D5Mit61 and D5Mit72 have been isolated. Cloned extremities of the YAC inserts were used to construct a 1.1-Mb contig, a 700-kb fragment of which was shown to contain the reeler locus. The integrity of the contig was verified by physical mapping on genomic DNA. The classical allele of the reeler mutation was associated with a 150-kb deletion between D5Mit61 and D5Mit72 , while no gross chromosomal anomaly was found in the Orléans allele. Candidate coding sequences were isolated to construct a preliminary transcriptional map of the reeler region. Cosmid clones mapping within the rl deletion revealed a large transcript of more than 11 kb, which was present in normal embryonic brain but barely detectable in homozygous rl Orl /rl Orl embryonic brain, suggesting strongly that it corresponds to the reeler transcript. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Genomics Elsevier

A YAC contig containing the reeler locus with preliminary characterization of candidate gene fragments

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Publisher
Elsevier
Copyright
Copyright © 1995 Elsevier Ltd
ISSN
0888-7543
eISSN
1089-8646
DOI
10.1016/0888-7543(95)80173-J
Publisher site
See Article on Publisher Site

Abstract

The reeler mutation in the mouse maps to proximal chromosome 5 and defines a key gene involved in brain development and evolution. No gene product is known, and the locus is currently being characterized by positional cloning. YAC clones corresponding to the closest markers D5Mit61 and D5Mit72 have been isolated. Cloned extremities of the YAC inserts were used to construct a 1.1-Mb contig, a 700-kb fragment of which was shown to contain the reeler locus. The integrity of the contig was verified by physical mapping on genomic DNA. The classical allele of the reeler mutation was associated with a 150-kb deletion between D5Mit61 and D5Mit72 , while no gross chromosomal anomaly was found in the Orléans allele. Candidate coding sequences were isolated to construct a preliminary transcriptional map of the reeler region. Cosmid clones mapping within the rl deletion revealed a large transcript of more than 11 kb, which was present in normal embryonic brain but barely detectable in homozygous rl Orl /rl Orl embryonic brain, suggesting strongly that it corresponds to the reeler transcript.

Journal

GenomicsElsevier

Published: Apr 10, 1995

References

  • Mechanisms of cortical development: A view from mutations in mice
    Caviness, V.S.; Rakic, P.
  • A genetic map of the mouse suitable for typing intraspecific crosses
    Dietrich, W.F.; Katz, H.; Lincoln, S.E.; Shin, H.S.; Friedman, J.; Dracopoli, N.; Lander, E.S.

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