A two-stage reverse dialysis in vitro dissolution testing method for passive targeted liposomes

A two-stage reverse dialysis in vitro dissolution testing method for passive targeted liposomes Article history: A novel two-stage reverse dialysis method has been developed for in vitro release testing of liposomal Received 29 November 2011 drug product with passive targeting characteristics. The first stage of the test is to mimic the circulation Received in revised form 11 January 2012 of liposomes in the body, whereas the second stage is to imitate the drug release process at the target. Accepted 13 January 2012 Buffer and surfactant solution were used during the first and second stages, respectively. For formula- Available online 23 January 2012 tions containing high phase transition temperature lipids and high cholesterol content, no drug leakage was observed during the first stage of test. In the second stage, however, formulations with different Keywords: compositions showed significant differences in terms of drug release rate, and discriminatory ability of Liposome the method was demonstrated. On comparing two different membrane diffusion techniques, dialysis and In vitro release reverse dialysis methods, the reverse dialysis method showed significantly lower variation, and there- Tenofovir fore is the preferred method. The developed in vitro release testing method should help to distinguish Dialysis Reverse dialysis formulations with varied compositions for quality control testing purposes. This two-stage reverse dial- Membrane diffusion http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Pharmaceutics Elsevier

A two-stage reverse dialysis in vitro dissolution testing method for passive targeted liposomes

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Publisher
Elsevier
Copyright
Copyright © 2012 Elsevier B.V.
ISSN
0378-5173
D.O.I.
10.1016/j.ijpharm.2012.01.030
Publisher site
See Article on Publisher Site

Abstract

Article history: A novel two-stage reverse dialysis method has been developed for in vitro release testing of liposomal Received 29 November 2011 drug product with passive targeting characteristics. The first stage of the test is to mimic the circulation Received in revised form 11 January 2012 of liposomes in the body, whereas the second stage is to imitate the drug release process at the target. Accepted 13 January 2012 Buffer and surfactant solution were used during the first and second stages, respectively. For formula- Available online 23 January 2012 tions containing high phase transition temperature lipids and high cholesterol content, no drug leakage was observed during the first stage of test. In the second stage, however, formulations with different Keywords: compositions showed significant differences in terms of drug release rate, and discriminatory ability of Liposome the method was demonstrated. On comparing two different membrane diffusion techniques, dialysis and In vitro release reverse dialysis methods, the reverse dialysis method showed significantly lower variation, and there- Tenofovir fore is the preferred method. The developed in vitro release testing method should help to distinguish Dialysis Reverse dialysis formulations with varied compositions for quality control testing purposes. This two-stage reverse dial- Membrane diffusion

Journal

International Journal of PharmaceuticsElsevier

Published: Apr 15, 2012

References

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