A technology platform for the fast production of monoclonal recombinant antibodies against plant proteins and peptides

A technology platform for the fast production of monoclonal recombinant antibodies against plant... The application of recombinant antibodies in plant biology research is limited because plant researchers have minimal access to high-quality phage display libraries. Therefore, we constructed a library of 1.3×10 10 clones displaying human single-chain variable fragments (scFvs) that is available to the academic community. The scFvs selected from the library against a diverse set of plant proteins showed moderate to high antigen-binding affinity together with high specificity. Moreover, to optimize an scFv as immunodetection agent, two expression systems that allow efficient production and purification of bivalent scFv–Fc and scFv–C κ ZIP fusion proteins were integrated. We are convinced that this antibody platform will further stimulate applications of recombinant antibodies such as the diagnostic detection or immunomodulation of specific antigens in plants. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Immunological Methods Elsevier

A technology platform for the fast production of monoclonal recombinant antibodies against plant proteins and peptides

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Publisher
Elsevier
Copyright
Copyright © 2004 Elsevier B.V.
ISSN
0022-1759
D.O.I.
10.1016/j.jim.2004.08.006
Publisher site
See Article on Publisher Site

Abstract

The application of recombinant antibodies in plant biology research is limited because plant researchers have minimal access to high-quality phage display libraries. Therefore, we constructed a library of 1.3×10 10 clones displaying human single-chain variable fragments (scFvs) that is available to the academic community. The scFvs selected from the library against a diverse set of plant proteins showed moderate to high antigen-binding affinity together with high specificity. Moreover, to optimize an scFv as immunodetection agent, two expression systems that allow efficient production and purification of bivalent scFv–Fc and scFv–C κ ZIP fusion proteins were integrated. We are convinced that this antibody platform will further stimulate applications of recombinant antibodies such as the diagnostic detection or immunomodulation of specific antigens in plants.

Journal

Journal of Immunological MethodsElsevier

Published: Nov 1, 2004

References

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