A single-plasmid reverse genetics system for the rescue of non-segmented negative-strand RNA viruses from cloned full-length cDNA

A single-plasmid reverse genetics system for the rescue of non-segmented negative-strand RNA... •A system to rescue Newcastle disease virus from a single plasmid is presented.•The system eliminates the need for co-transfection of helper plasmids.•It relies on the insertion of T7 promoter sequences in the viral cDNA.•The T7 promoters allow the expression of essential viral replication proteins.•The system may be applicable to all non-segmented negative-strand RNA viruses. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

A single-plasmid reverse genetics system for the rescue of non-segmented negative-strand RNA viruses from cloned full-length cDNA

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2017.07.008
Publisher site
See Article on Publisher Site

Abstract

•A system to rescue Newcastle disease virus from a single plasmid is presented.•The system eliminates the need for co-transfection of helper plasmids.•It relies on the insertion of T7 promoter sequences in the viral cDNA.•The T7 promoters allow the expression of essential viral replication proteins.•The system may be applicable to all non-segmented negative-strand RNA viruses.

Journal

Journal of Virological MethodsElsevier

Published: Oct 1, 2017

References

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