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) by incubation with fluorescent ‘trifunctional’ probe 4 (Fig 1; 5 μM) in the presence of CuSO4 (1 mM), sodium ascorbate (2 mM) and tris-hydroxypropyltriazolylmethylamine (THPTA, 2 mM) for 1h at room temperature ...
permeabilization To investigate apigenin-mediated membrane disruption, DiSC3(5), a potentiometric fluorescent probe , was used. Accumulation of the probe in the cell membrane leads to self-quenching of its ...
, flavone synthase; IFS, isoflavone synthase; LAR, leucoanthocyanidin reductase; ANS, anthocyanidin synthase; ANR, anthocyanidin reductase; GT, glucosyltransferase. Figure 1. Open in new tabDownload slide ...
in Arabidopsis (Arabidopsis thaliana), NCED3 plays a key role in ABA biosynthesis during water deficit and salt stress (Iuchi et al., 2001; Tan et al., 2003; Nambara and Marion-Poll, 2005; Barrero et al., 2006 ...
is not fully soluble in water , the exact final concentration in the agar medium is not certain. For NPA treatment, seedlings were grown for 5 d in a vertical orientation on half-strength MS medium, as described ...
truncatula in conditions with no improved Pi status and compared them with those induced by high-Pi treatment in nonmycorrhized plants. Microarray- based genome-wide profiling indicated up-regulation ...
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