A sensitive flow cytometric method for multi-parametric analysis of microRNA, messenger RNA and protein in single cells

A sensitive flow cytometric method for multi-parametric analysis of microRNA, messenger RNA and... •PrimeFlow RNA Assay allows for multiplex analysis of microRNA, mRNA and protein.•The detection limit is ∼10 copies per cell for mRNA and ∼20 copies for microRNA.•The assay revealed heterogeneity in polarized macrophage subsets.•Unique microRNA profiles were detected in mouse peritoneal cell populations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Methods Elsevier

A sensitive flow cytometric method for multi-parametric analysis of microRNA, messenger RNA and protein in single cells

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Inc.
ISSN
1046-2023
eISSN
1095-9130
D.O.I.
10.1016/j.ymeth.2017.12.016
Publisher site
See Article on Publisher Site

Abstract

•PrimeFlow RNA Assay allows for multiplex analysis of microRNA, mRNA and protein.•The detection limit is ∼10 copies per cell for mRNA and ∼20 copies for microRNA.•The assay revealed heterogeneity in polarized macrophage subsets.•Unique microRNA profiles were detected in mouse peritoneal cell populations.

Journal

MethodsElsevier

Published: Feb 1, 2018

References

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