A renaissance for the pioneering 16S rRNA gene

A renaissance for the pioneering 16S rRNA gene Culture-independent molecular surveys using the 16S rRNA gene have become a mainstay for characterizing microbial community structure over the past quarter century. More recently this approach has been overshadowed by metagenomics, which provides a global overview of a community’s functional potential rather than just an inventory of its inhabitants. However, the pioneering 16S rRNA gene is making a comeback in its own right thanks to a number of methodological advancements including higher resolution (more sequences), analysis of multiple related samples (e.g. spatial and temporal series) and improved metadata, and use of metadata. The standard conclusion that microbial ecosystems are remarkably complex and diverse is now being replaced by detailed insights into microbial ecology and evolution based only on this one historically important marker gene. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Current Opinion in Microbiology Elsevier

A renaissance for the pioneering 16S rRNA gene

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Publisher
Elsevier
Copyright
Copyright © 2008 Elsevier Ltd
ISSN
1369-5274
DOI
10.1016/j.mib.2008.09.011
Publisher site
See Article on Publisher Site

Abstract

Culture-independent molecular surveys using the 16S rRNA gene have become a mainstay for characterizing microbial community structure over the past quarter century. More recently this approach has been overshadowed by metagenomics, which provides a global overview of a community’s functional potential rather than just an inventory of its inhabitants. However, the pioneering 16S rRNA gene is making a comeback in its own right thanks to a number of methodological advancements including higher resolution (more sequences), analysis of multiple related samples (e.g. spatial and temporal series) and improved metadata, and use of metadata. The standard conclusion that microbial ecosystems are remarkably complex and diverse is now being replaced by detailed insights into microbial ecology and evolution based only on this one historically important marker gene.

Journal

Current Opinion in MicrobiologyElsevier

Published: Oct 1, 2008

References

  • Separating the determinants of phylogenetic community structure
    Helmus, M.R.; Savage, K.; Diebel, M.W.; Maxted, J.T.; Ives, A.R.
  • The minimum information about a genome sequence (MIGS) specification
    Field, D.; Garrity, G.; Gray, T.; Morrison, N.; Selengut, J.; Sterk, P.; Tatusova, T.; Thomson, N.; Allen, M.J.; Angiuoli, S.V.
  • RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models
    Stamatakis, A.

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