A rapid method for titration of ascovirus infectivity

A rapid method for titration of ascovirus infectivity Journal of Virological Methods 255 (2018) 101–106 Contents lists available at ScienceDirect Journal of Virological Methods journal homepage: www.elsevier.com/locate/jviromet ⁎ ⁎⁎ a b a b a, a,c, Ningning Han , Zishu Chen , Hu Wan , Guohua Huang , Jianhong Li , Byung Rae Jin Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China Institute of Virology, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Republic of Korea ARTICLE I NFO ABSTRACT Keywords: Ascoviruses are a recently described family and the traditional plaque assay and end-point PCR assay have been Ascoviruses used for their titration. However, these two methods are time-consuming and inaccurate to titrate ascoviruses. In Titration the present study, a quick method for the determination of the titer of ascovirus stocks was developed based on Flow cytometry ascovirus-induced apoptosis in infected insect cells. Briefly, cells infected with serial dilutions of virus Apoptosis −2 −10 (10 –10 ) for 24 h were stained with trypan blue. The stained cells were counted, and the percentage of nonviable cells was calculated. The http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

A rapid method for titration of ascovirus infectivity

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2018.02.011
Publisher site
See Article on Publisher Site

Abstract

Journal of Virological Methods 255 (2018) 101–106 Contents lists available at ScienceDirect Journal of Virological Methods journal homepage: www.elsevier.com/locate/jviromet ⁎ ⁎⁎ a b a b a, a,c, Ningning Han , Zishu Chen , Hu Wan , Guohua Huang , Jianhong Li , Byung Rae Jin Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China Institute of Virology, College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Republic of Korea ARTICLE I NFO ABSTRACT Keywords: Ascoviruses are a recently described family and the traditional plaque assay and end-point PCR assay have been Ascoviruses used for their titration. However, these two methods are time-consuming and inaccurate to titrate ascoviruses. In Titration the present study, a quick method for the determination of the titer of ascovirus stocks was developed based on Flow cytometry ascovirus-induced apoptosis in infected insect cells. Briefly, cells infected with serial dilutions of virus Apoptosis −2 −10 (10 –10 ) for 24 h were stained with trypan blue. The stained cells were counted, and the percentage of nonviable cells was calculated. The

Journal

Journal of Virological MethodsElsevier

Published: May 1, 2018

References

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