Article history: Background: Elevated intracranial pressure (ICP) accompanying a number of neurological emergencies Received 28 March 2017 is poorly understood, and lacks a model to determine cellular pathophysiology. This limits our ability to Received in revised form 2 August 2017 identify cellular and molecular biomarkers associated with the pathological progression from physiologic Accepted 3 October 2017 to pathologic ICP. Available online 6 October 2017 New method: We developed an ex vivo model of pressure-induced brain injury, which combines 3D neural cell cultures and a newly developed Pressure Controlled Cell Culture Incubator (PC I). Human astrocytes Keywords: and neurons maintained in 3D peptide-conjugated alginate hydrogels were subjected to pressures that Hydrocephalus mimic both physiologic and pathologic levels of ICP for up to 48 h to evaluate the earliest impacts of Traumatic brain injury isolated pressure on cellular viability and quantify early indicators of pressure-induced cellular injury. Pressure injury Results: Compared to control cell cultures grown under physiologic pressure, sustained pathologic pres- 3D model Engineered neural tissue sure exposure increased the release of intracellular ATP in a cell-speciﬁc manner. Eighteen hours of sustained pressure resulted in increased ATP release from neurons but not astrocytes. Comparison with existing methods: Cell culture incubators
Journal of Neuroscience Methods – Elsevier
Published: Jan 1, 2018
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