Bioresource Technology 249 (2018) 1000–1008 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech A novel protein tyrosine phosphatase like phytase from Lactobacillus fermentum NKN51: Cloning, characterization and application in mineral release for food technology applications a,1 a,1 a b a, Rekha Sharma , Piyush Kumar , Vandana Kaushal , Rahul Das , Naveen Kumar Navani Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India Department of Biological Sciences, Indian Institute of Science Education and Research, Kolkata 741246, India GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: A novel protein tyrosine phosphatase like phytase (PTPLP), designated as PhyLf from probiotic bacterium Protein tyrosine phosphatase like phytase Lactobacillus fermentum NKN51 was identiﬁed, cloned, expressed and characterized. The recombinant PhyLf Lactobacillus fermentum NKN51 showed speciﬁc activity of 174.5 U/mg. PhyLf exhibited strict speciﬁcity towards phytate and optimum tem- Dephytinization perature at 60 °C, pH 5.0 and ionic strength of 100 mM. K and K of PhyLf for phytate were 0.773 mM and m cat Gastrointestinal conditions −1 84.31 s , respectively. PhyLf exhibited high resistance against oxidative inactivation. PhyLf shares no Bioaccessibility homology, sans the active site with reported PTLPs, warranting classiﬁcation as a new subclass.
Bioresource Technology – Elsevier
Published: Feb 1, 2018
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