A novel bacteriocin DY4-2 produced by Lactobacillus plantarum from cutlassfish and its application as bio-preservative for the control of Pseudomonas fluorescens in fresh turbot (Scophthalmus maximus) fillets

A novel bacteriocin DY4-2 produced by Lactobacillus plantarum from cutlassfish and its... A novel bacteriocin DY4-2 was produced by strain DY4-2 which was isolated from cutlassfish (Trichiurus lepturus) and identified as Lactobacillus plantarum. This bacteriocin reached the maximum production at stationary phase after 24 h incubation. It was purified by ethyl acetate extraction followed by ultrafiltration, Sephadex G25 chromatography and high-performance liquid chromatography. The molecular mass of bacteriocin DY4-2 was determined as 1465 Da by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. The amino acid sequence of bacteriocin DY4-2 was predicted to be HAQIGMTMNGSFR by MALDI-TOF-MS/MS. Bacteriocin DY4-2 was heat-stable (30 min at 121 °C) and kept active at the pH range of 2.5–5.5, but was sensitive to proteolytic enzymes. The mode of action of bacteriocin DY4-2 was bactericidal. It exhibited broad spectrum antimicrobial activity against fish pathogenic and spoilage bacteria, such as Pseudomonas fluorescens, P. aeruginosa, Vibrio parahaemolyticus, Aeromonas sobria and Listeria monocytogenes. The addition of partially purified bacteriocin DY4-2 in turbot fillets reduced the number of P. fluorescens by 2.7 log units at 4 °C storage for 12 days. Moreover, compared to the control, a significant decline in total viable counts and total volatile base nitrogen was observed in bacteriocin DY4-2 treated samples. Theses results suggested that bacteriocin DY4-2 had potential to act as seafood bio-preservative. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Food Control Elsevier

A novel bacteriocin DY4-2 produced by Lactobacillus plantarum from cutlassfish and its application as bio-preservative for the control of Pseudomonas fluorescens in fresh turbot (Scophthalmus maximus) fillets

Loading next page...
 
/lp/elsevier/a-novel-bacteriocin-dy4-2-produced-by-lactobacillus-plantarum-from-rGd2lsVn4S
Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier Ltd
ISSN
0956-7135
eISSN
1873-7129
D.O.I.
10.1016/j.foodcont.2018.02.002
Publisher site
See Article on Publisher Site

Abstract

A novel bacteriocin DY4-2 was produced by strain DY4-2 which was isolated from cutlassfish (Trichiurus lepturus) and identified as Lactobacillus plantarum. This bacteriocin reached the maximum production at stationary phase after 24 h incubation. It was purified by ethyl acetate extraction followed by ultrafiltration, Sephadex G25 chromatography and high-performance liquid chromatography. The molecular mass of bacteriocin DY4-2 was determined as 1465 Da by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. The amino acid sequence of bacteriocin DY4-2 was predicted to be HAQIGMTMNGSFR by MALDI-TOF-MS/MS. Bacteriocin DY4-2 was heat-stable (30 min at 121 °C) and kept active at the pH range of 2.5–5.5, but was sensitive to proteolytic enzymes. The mode of action of bacteriocin DY4-2 was bactericidal. It exhibited broad spectrum antimicrobial activity against fish pathogenic and spoilage bacteria, such as Pseudomonas fluorescens, P. aeruginosa, Vibrio parahaemolyticus, Aeromonas sobria and Listeria monocytogenes. The addition of partially purified bacteriocin DY4-2 in turbot fillets reduced the number of P. fluorescens by 2.7 log units at 4 °C storage for 12 days. Moreover, compared to the control, a significant decline in total viable counts and total volatile base nitrogen was observed in bacteriocin DY4-2 treated samples. Theses results suggested that bacteriocin DY4-2 had potential to act as seafood bio-preservative.

Journal

Food ControlElsevier

Published: Jul 1, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off