A novel antibody-based approach to detect the functional ERCC1-202 isoform

A novel antibody-based approach to detect the functional ERCC1-202 isoform •ERCC1-202 and ERCC1-204 isoforms, but not other isoforms, interact properly with XPF in vitro.•We developed new monoclonal antibodies specific to the ERCC1-202/XPF and ERCC1-204/XPF heterodimers.•The antibodies perform well in ELISA, IF and IP applications, but not in WB nor in IHC on FFPE tissues.•We detect specifically ERCC1-202 using these antibodies in a proximity ligation assay with an anti-ERCC1 antibody unable to recognize ERCC1-204. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png DNA Repair Elsevier

A novel antibody-based approach to detect the functional ERCC1-202 isoform

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Publisher
Elsevier
Copyright
Copyright © 2018 Elsevier B.V.
ISSN
1568-7864
D.O.I.
10.1016/j.dnarep.2018.02.002
Publisher site
See Article on Publisher Site

Abstract

•ERCC1-202 and ERCC1-204 isoforms, but not other isoforms, interact properly with XPF in vitro.•We developed new monoclonal antibodies specific to the ERCC1-202/XPF and ERCC1-204/XPF heterodimers.•The antibodies perform well in ELISA, IF and IP applications, but not in WB nor in IHC on FFPE tissues.•We detect specifically ERCC1-202 using these antibodies in a proximity ligation assay with an anti-ERCC1 antibody unable to recognize ERCC1-204.

Journal

DNA RepairElsevier

Published: Apr 1, 2018

References

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