A new real-time RT-qPCR assay for the detection, subtyping and quantification of human respiratory syncytial viruses positive- and negative-sense RNAs

A new real-time RT-qPCR assay for the detection, subtyping and quantification of human... •New quantitative RT-qPCR assays for RSV-A and -B were developed.•Both assays allow rapid detection, quantification and subtyping of RSV.•The efficiency test was validated with in vitro transcripts and clinical samples.•Comparable sensitivity was observed with RSV positive and negative sense RNAs.•The assays provide a useful tool for both RSV diagnostics and research. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

A new real-time RT-qPCR assay for the detection, subtyping and quantification of human respiratory syncytial viruses positive- and negative-sense RNAs

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Publisher
Elsevier
Copyright
Copyright © 2016 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2016.05.004
Publisher site
See Article on Publisher Site

Abstract

•New quantitative RT-qPCR assays for RSV-A and -B were developed.•Both assays allow rapid detection, quantification and subtyping of RSV.•The efficiency test was validated with in vitro transcripts and clinical samples.•Comparable sensitivity was observed with RSV positive and negative sense RNAs.•The assays provide a useful tool for both RSV diagnostics and research.

Journal

Journal of Virological MethodsElsevier

Published: Sep 1, 2016

References

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