A narrow window for rescuing cells by the inhibition of calcium influx and the importance of influx route in rat cortical neuronal cell death induced by glutamate

A narrow window for rescuing cells by the inhibition of calcium influx and the importance of... A rescue window for glutamate-insulted cells with regard to Ca 2+ influx was first investigated. The addition of EGTA, an impermeable calcium chelator to the culture within 5 min after the beginning of glutamate stimulation potently suppressed the neuronal cell death examined at 22 h. The effect of EGTA on rescuing cells decreased with the time delay of its addition to the system. MK-801, an antagonist of N -methyl- d -asparate (NMDA) receptor channels also inhibited the neuronal cell death in a similar manner to EGTA, suggesting Ca 2+ influx up to 5 min after the insult determined the fate of cells. But we also demonstrated that the elevated intracellular Ca 2+ did not always induce neurotoxicity. High concentration of potassium chloride plus FPL64176, an agonist of L-type Ca 2+ channels did not induce neuronal cell death, even though their combination elicited equal elevation of intracellular Ca 2+ to that by toxic concentration of glutamate, demonstrating that locally elevated intracellular Ca 2+ around NMDA receptors is important in the induction of neuronal cell death. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neuroscience Letters Elsevier

A narrow window for rescuing cells by the inhibition of calcium influx and the importance of influx route in rat cortical neuronal cell death induced by glutamate

Loading next page...
 
/lp/elsevier/a-narrow-window-for-rescuing-cells-by-the-inhibition-of-calcium-influx-LM7909jP7P
Publisher
Elsevier
Copyright
Copyright © 2001 Elsevier Science Ireland Ltd
ISSN
0304-3940
D.O.I.
10.1016/S0304-3940(01)01742-6
Publisher site
See Article on Publisher Site

Abstract

A rescue window for glutamate-insulted cells with regard to Ca 2+ influx was first investigated. The addition of EGTA, an impermeable calcium chelator to the culture within 5 min after the beginning of glutamate stimulation potently suppressed the neuronal cell death examined at 22 h. The effect of EGTA on rescuing cells decreased with the time delay of its addition to the system. MK-801, an antagonist of N -methyl- d -asparate (NMDA) receptor channels also inhibited the neuronal cell death in a similar manner to EGTA, suggesting Ca 2+ influx up to 5 min after the insult determined the fate of cells. But we also demonstrated that the elevated intracellular Ca 2+ did not always induce neurotoxicity. High concentration of potassium chloride plus FPL64176, an agonist of L-type Ca 2+ channels did not induce neuronal cell death, even though their combination elicited equal elevation of intracellular Ca 2+ to that by toxic concentration of glutamate, demonstrating that locally elevated intracellular Ca 2+ around NMDA receptors is important in the induction of neuronal cell death.

Journal

Neuroscience LettersElsevier

Published: May 18, 2001

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off