A major bristle QTL from a selected population of Drosophila uncovers the zinc-finger transcription factor Poils-au-dos, a repressor of achaete–scute

A major bristle QTL from a selected population of Drosophila uncovers the zinc-finger... Traditional screens aiming at identifying genes regulating development have relied on mutagenesis. Here, we describe a new gene involved in bristle development, identified through the use of natural variation and selection. Drosophila melanogaster bears a pattern of 11 macrochaetes per heminotum. From a population initially sampled in Marrakech, a strain was selected for an increased number of thoracic macrochaetes. Using recombination and single nucleotide polymorphisms, the factor responsible was mapped to a single locus on the third chromosome, poils au dos , that encodes a zinc-finger-ZAD protein. The original, as well as new, presumed null, alleles of poils au dos , is associated with ectopic achaete–scute expression that results in the additional bristles. This suggests a possible role for Poils au dos as a repressor of achaete and scute . Ectopic expression appears to be independent of the activity of known cis- regulatory enhancer sequences at the achaete–scute complex that mediate activation at specific sites on the notum. The target sequences for Poils au dos activity were mapped to a 14 kb region around scute . In addition, we show that pad interacts synergistically with the repressor hairy and with Dpp signaling in posterior and anterior regions of the notum, respectively. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Developmental Biology Elsevier

A major bristle QTL from a selected population of Drosophila uncovers the zinc-finger transcription factor Poils-au-dos, a repressor of achaete–scute

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Publisher
Elsevier
Copyright
Copyright © 2005 Elsevier Inc.
ISSN
0012-1606
eISSN
1095-564X
D.O.I.
10.1016/j.ydbio.2005.09.032
Publisher site
See Article on Publisher Site

Abstract

Traditional screens aiming at identifying genes regulating development have relied on mutagenesis. Here, we describe a new gene involved in bristle development, identified through the use of natural variation and selection. Drosophila melanogaster bears a pattern of 11 macrochaetes per heminotum. From a population initially sampled in Marrakech, a strain was selected for an increased number of thoracic macrochaetes. Using recombination and single nucleotide polymorphisms, the factor responsible was mapped to a single locus on the third chromosome, poils au dos , that encodes a zinc-finger-ZAD protein. The original, as well as new, presumed null, alleles of poils au dos , is associated with ectopic achaete–scute expression that results in the additional bristles. This suggests a possible role for Poils au dos as a repressor of achaete and scute . Ectopic expression appears to be independent of the activity of known cis- regulatory enhancer sequences at the achaete–scute complex that mediate activation at specific sites on the notum. The target sequences for Poils au dos activity were mapped to a 14 kb region around scute . In addition, we show that pad interacts synergistically with the repressor hairy and with Dpp signaling in posterior and anterior regions of the notum, respectively.

Journal

Developmental BiologyElsevier

Published: Dec 1, 2005

References

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