A highly attenuated rabies virus HEP-Flury strain reverts to virulent by single amino acid substitution to arginine at position 333 in glycoprotein

A highly attenuated rabies virus HEP-Flury strain reverts to virulent by single amino acid... An amino acid at position 333 in the glycoprotein of several fixed rabies virus strains is responsible for the pathogenicity in adult mice. Substitution of arginine at this position largely reduces the viral pathogenicity in adult mice. Attenuation by this single amino acid substitution has been established by using escape mutants selected by monoclonal antibodies and point-mutated virus generated by reverse-genetics. A highly attenuated HEP-Flury strain, which was selected by serial passages in cell cultures, has glutamine at this position. In this study, a point-mutated rHEP 333 R virus, having arginine at position 333, was generated and examined for the responsibility of this substitution in rabies pathogenicity. The rHEP 333 R acquired an ability to spread and propagate in mouse brain but the parental rHEP did not. The pathogenicity of rHEP 333 R to adult mice by intracerebral inoculation largely increased. We confirmed that an arginine at position 333 contributed to reversion of the pathogenicity in a highly attenuated HEP-Flury strain. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Virus Research Elsevier

A highly attenuated rabies virus HEP-Flury strain reverts to virulent by single amino acid substitution to arginine at position 333 in glycoprotein

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Publisher
Elsevier
Copyright
Copyright © 2006 Elsevier B.V.
ISSN
0168-1702
D.O.I.
10.1016/j.virusres.2006.01.014
Publisher site
See Article on Publisher Site

Abstract

An amino acid at position 333 in the glycoprotein of several fixed rabies virus strains is responsible for the pathogenicity in adult mice. Substitution of arginine at this position largely reduces the viral pathogenicity in adult mice. Attenuation by this single amino acid substitution has been established by using escape mutants selected by monoclonal antibodies and point-mutated virus generated by reverse-genetics. A highly attenuated HEP-Flury strain, which was selected by serial passages in cell cultures, has glutamine at this position. In this study, a point-mutated rHEP 333 R virus, having arginine at position 333, was generated and examined for the responsibility of this substitution in rabies pathogenicity. The rHEP 333 R acquired an ability to spread and propagate in mouse brain but the parental rHEP did not. The pathogenicity of rHEP 333 R to adult mice by intracerebral inoculation largely increased. We confirmed that an arginine at position 333 contributed to reversion of the pathogenicity in a highly attenuated HEP-Flury strain.

Journal

Virus ResearchElsevier

Published: Aug 1, 2006

References

  • An improved method for recovering rabies virus from cloned cDNA
    Inoue, K.; Shoji, Y.; Kurane, I.; Iijima, T.; Sakai, T.; Morimoto, K.
  • Cross-reactive antigenicity of nucleoproteins of lyssaviruses recognized by a monospecific antirabies virus nucleoprotein antiserum on paraffin sections of formalin-fixed tissues
    Inoue, S.; Sato, Y.; Hasegawa, H.; Noguchi, A.; Yamada, A.; Kurata, T.; Iwasaki, T.
  • Rabies as a transneuronal tracer of circuits in the central nervous system
    Kelly, R.M.; Strick, P.L.
  • Is the acetylcholine receptor a rabies virus receptor?
    Lentz, T.L.; Burrage, T.G.; Smith, A.L.; Crick, J.; Tignor, G.H.
  • Rabies virus glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous system after peripheral delivery
    Mazarakis, N.D.; Azzouz, M.; Rohll, J.B.; Ellard, F.M.; Wilkes, F.J.; Olsen, A.L.; Carter, E.E.; Barber, R.D.; Baban, D.F.; Kingsman, S.M.; Kingsman, A.J.; O’Malley, K.; Mitrophanous, K.A.
  • Low-affinity nerve-growth factor receptor (P75NTR) can serve as a receptor for rabies virus
    Tuffereau, C.; Benejean, J.; Blondel, D.; Kieffer, B.; Flamand, A.
  • Suppression of cell-mediated immunity by street rabies virus
    Wiktor, T.J.; Doherty, P.C.; Koprowski, H.

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