A high content imaging flow cytometry approach to study mitochondria in T cells: MitoTracker Green FM dye concentration optimization

A high content imaging flow cytometry approach to study mitochondria in T cells: MitoTracker... •IFC allows quantification of the mitochondria in T cells in a high throughput manner.•50 nM is the optimum concentration to label mitochondria in T cells using IFC.•Controls, dye concentration and acquisition parameters are crucial for reproducibility.•The Raw Max Pixel values can be used to standardize dye concentration and voltage. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Methods Elsevier

A high content imaging flow cytometry approach to study mitochondria in T cells: MitoTracker Green FM dye concentration optimization

Loading next page...
 
/lp/elsevier/a-high-content-imaging-flow-cytometry-approach-to-study-mitochondria-DSgxbFOnj1
Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Inc.
ISSN
1046-2023
eISSN
1095-9130
D.O.I.
10.1016/j.ymeth.2017.11.015
Publisher site
See Article on Publisher Site

Abstract

•IFC allows quantification of the mitochondria in T cells in a high throughput manner.•50 nM is the optimum concentration to label mitochondria in T cells using IFC.•Controls, dye concentration and acquisition parameters are crucial for reproducibility.•The Raw Max Pixel values can be used to standardize dye concentration and voltage.

Journal

MethodsElsevier

Published: Feb 1, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off