A field based detection method for Rose rosette virus using isothermal probe-based Reverse transcription-recombinase polymerase amplification assay

A field based detection method for Rose rosette virus using isothermal probe-based Reverse... •Developed a novel and rapid (20min) RT-exoRPA assay for the detection of Rose rosette virus (RRV).•The RT-exoRPA primers/probe was highly specific to RRV.•The assay was highly sensitive, detecting up to 1 fg of virus.•A rapid viral RNA extraction procedure for RRV (<5min) was developed from different tissue sources.•The assay detected RRV from different tissue sources of infected plants, as well as from different rose varieties. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Virological Methods Elsevier

A field based detection method for Rose rosette virus using isothermal probe-based Reverse transcription-recombinase polymerase amplification assay

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier B.V.
ISSN
0166-0934
eISSN
1879-0984
D.O.I.
10.1016/j.jviromet.2017.05.019
Publisher site
See Article on Publisher Site

Abstract

•Developed a novel and rapid (20min) RT-exoRPA assay for the detection of Rose rosette virus (RRV).•The RT-exoRPA primers/probe was highly specific to RRV.•The assay was highly sensitive, detecting up to 1 fg of virus.•A rapid viral RNA extraction procedure for RRV (<5min) was developed from different tissue sources.•The assay detected RRV from different tissue sources of infected plants, as well as from different rose varieties.

Journal

Journal of Virological MethodsElsevier

Published: Sep 1, 2017

References

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