A continuous cell line from the cultured marine fish gilt-head seabream ( Sparus aurata L.)

A continuous cell line from the cultured marine fish gilt-head seabream ( Sparus aurata L.) Conspecific cell lines provide an efficient experimental system with many applications for improving the productivity of commercial fish species. A continuous cell line SAF-1 has been developed from fin tissues of an adult gilt-head seabream ( Sparus aurata ) without immortalising treatments. The cells grow in DME-F12 basal medium supplemented with 5% foetal bovine serum at the optimal temperature of 25 °C and have been sub-cultured approximately 70 times over a 1.5 year period. They show a fibroblast-like morphology, high efficiency of plating, doubling time of 2 days and high survival after being frozen. Karyotype and DNA content of the cell line reveal a mostly euploid and rather stable cell population, which is of great interest for cytogenetic studies. This cell line has been shown to be susceptible to several fish viruses and to bacterial extracellular products. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Aquaculture Elsevier

A continuous cell line from the cultured marine fish gilt-head seabream ( Sparus aurata L.)

Aquaculture, Volume 150 (1) – Apr 15, 1997

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Publisher
Elsevier
Copyright
Copyright © 1997 Elsevier Ltd
ISSN
0044-8486
D.O.I.
10.1016/S0044-8486(96)01469-X
Publisher site
See Article on Publisher Site

Abstract

Conspecific cell lines provide an efficient experimental system with many applications for improving the productivity of commercial fish species. A continuous cell line SAF-1 has been developed from fin tissues of an adult gilt-head seabream ( Sparus aurata ) without immortalising treatments. The cells grow in DME-F12 basal medium supplemented with 5% foetal bovine serum at the optimal temperature of 25 °C and have been sub-cultured approximately 70 times over a 1.5 year period. They show a fibroblast-like morphology, high efficiency of plating, doubling time of 2 days and high survival after being frozen. Karyotype and DNA content of the cell line reveal a mostly euploid and rather stable cell population, which is of great interest for cytogenetic studies. This cell line has been shown to be susceptible to several fish viruses and to bacterial extracellular products.

Journal

AquacultureElsevier

Published: Apr 15, 1997

References

  • Short-term cell culture technique for obtaining chromosomes in marine and freshwater fish
    Alvarez, M.C.; Otis, J.; Amores, A.; Guise, K.

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