A comparative study of immunomodulation produced by in vitro exposure to delta opioid receptor agonist peptides

A comparative study of immunomodulation produced by in vitro exposure to delta opioid receptor... The present study assessed the direct immunomodulatory effect of a panel of synthetic peptides exhibiting δ-opioid receptor agonist activity. Murine splenic lymphocytes and peritoneal macrophages were cultured in vitro with peptides at concentrations of 0.00001–10 μ M . Assessment was made of B-cell function by quantitating cellular proliferation, T-cell function by measuring cytokine production, natural immunity by quantitating basal and cytokine-augmented natural killer (NK) cell activity, and macrophage function by production of IL-6. These peptides had minimal effects on B-cell proliferation at any concentration examined. In comparison, enhancement of cytokine production by T-helper cells occurred following exposure to several of the compounds, to a significant extent with DPDPE, DPDPE-trifluoroacetate, or deltorphin-1 and most pronounced at concentrations between 0.00001 and 0.1 μ M . Likewise, IL-6 production by macrophages was significantly augmented by exposure to these three peptides. NK cell function was significantly enhanced by in vitro exposure to several of the peptides, with enhancement generally noted at concentrations between 0.00001 and 0.01 μ M . However, some of the peptides (most notably DADLE) greatly suppressed NK cell activity. These data suggest that δ opioid agonists are broadly immunomostimulatory. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Peptides Elsevier

A comparative study of immunomodulation produced by in vitro exposure to delta opioid receptor agonist peptides

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Publisher
Elsevier
Copyright
Copyright © 1996 Elsevier Ltd
ISSN
0196-9781
DOI
10.1016/0196-9781(95)02051-9
Publisher site
See Article on Publisher Site

Abstract

The present study assessed the direct immunomodulatory effect of a panel of synthetic peptides exhibiting δ-opioid receptor agonist activity. Murine splenic lymphocytes and peritoneal macrophages were cultured in vitro with peptides at concentrations of 0.00001–10 μ M . Assessment was made of B-cell function by quantitating cellular proliferation, T-cell function by measuring cytokine production, natural immunity by quantitating basal and cytokine-augmented natural killer (NK) cell activity, and macrophage function by production of IL-6. These peptides had minimal effects on B-cell proliferation at any concentration examined. In comparison, enhancement of cytokine production by T-helper cells occurred following exposure to several of the compounds, to a significant extent with DPDPE, DPDPE-trifluoroacetate, or deltorphin-1 and most pronounced at concentrations between 0.00001 and 0.1 μ M . Likewise, IL-6 production by macrophages was significantly augmented by exposure to these three peptides. NK cell function was significantly enhanced by in vitro exposure to several of the peptides, with enhancement generally noted at concentrations between 0.00001 and 0.01 μ M . However, some of the peptides (most notably DADLE) greatly suppressed NK cell activity. These data suggest that δ opioid agonists are broadly immunomostimulatory.

Journal

PeptidesElsevier

Published: Jan 1, 1996

References

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