A closed-loop strategy for endoglucanase production using sugarcane bagasse liquefied by a home-made enzymatic cocktail

A closed-loop strategy for endoglucanase production using sugarcane bagasse liquefied by a... Bioresource Technology 249 (2018) 976–982 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech A closed-loop strategy for endoglucanase production using sugarcane bagasse liquefied by a home-made enzymatic cocktail a,b a a,b, Paula Squinca , Alberto C. Badino , Cristiane S. Farinas Graduate Program of Chemical Engineering, Federal University of São Carlos, C.P. 676, 13565-905 São Carlos, SP, Brazil Embrapa Instrumentation, Rua XV de Novembro 1452, 13561-206 São Carlos, SP, Brazil GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: Use of the same lignocellulosic biomass as feedstock for enzymes and ethanol production has been suggested as a Liquefaction lower cost option in future biorefineries. Here, we propose a closed-loop strategy to produce the cellulolytic Cellulases enzymes required for biomass hydrolysis using sugarcane bagasse liquefied by a home-made enzymatic cocktail Enzymes as carbon source and inducer. The fed-batch liquefaction conditions were firstly evaluated using commercial Biorefinery enzymes. Subsequently, the effects of different liquefied materials and solids loadings on endoglucanase pro- Biomass hydrolysis duction by Aspergillus niger cultivated in submerged fermentation were investigated. The liquefied bagasse produced using the home-made cocktail was more favorable for endoglucanase production, resulting in im- provement up to 17%, compared to bagasse liquefied by http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Bioresource Technology Elsevier

A closed-loop strategy for endoglucanase production using sugarcane bagasse liquefied by a home-made enzymatic cocktail

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Publisher
Elsevier
Copyright
Copyright © 2017 Elsevier Ltd
ISSN
0960-8524
D.O.I.
10.1016/j.biortech.2017.10.107
Publisher site
See Article on Publisher Site

Abstract

Bioresource Technology 249 (2018) 976–982 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech A closed-loop strategy for endoglucanase production using sugarcane bagasse liquefied by a home-made enzymatic cocktail a,b a a,b, Paula Squinca , Alberto C. Badino , Cristiane S. Farinas Graduate Program of Chemical Engineering, Federal University of São Carlos, C.P. 676, 13565-905 São Carlos, SP, Brazil Embrapa Instrumentation, Rua XV de Novembro 1452, 13561-206 São Carlos, SP, Brazil GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: Use of the same lignocellulosic biomass as feedstock for enzymes and ethanol production has been suggested as a Liquefaction lower cost option in future biorefineries. Here, we propose a closed-loop strategy to produce the cellulolytic Cellulases enzymes required for biomass hydrolysis using sugarcane bagasse liquefied by a home-made enzymatic cocktail Enzymes as carbon source and inducer. The fed-batch liquefaction conditions were firstly evaluated using commercial Biorefinery enzymes. Subsequently, the effects of different liquefied materials and solids loadings on endoglucanase pro- Biomass hydrolysis duction by Aspergillus niger cultivated in submerged fermentation were investigated. The liquefied bagasse produced using the home-made cocktail was more favorable for endoglucanase production, resulting in im- provement up to 17%, compared to bagasse liquefied by

Journal

Bioresource TechnologyElsevier

Published: Feb 1, 2018

References

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