Bioresource Technology 249 (2018) 976–982 Contents lists available at ScienceDirect Bioresource Technology journal homepage: www.elsevier.com/locate/biortech A closed-loop strategy for endoglucanase production using sugarcane bagasse liqueﬁed by a home-made enzymatic cocktail a,b a a,b, Paula Squinca , Alberto C. Badino , Cristiane S. Farinas Graduate Program of Chemical Engineering, Federal University of São Carlos, C.P. 676, 13565-905 São Carlos, SP, Brazil Embrapa Instrumentation, Rua XV de Novembro 1452, 13561-206 São Carlos, SP, Brazil GR APHICAL A BSTRACT ARTICLE I NFO ABSTRACT Keywords: Use of the same lignocellulosic biomass as feedstock for enzymes and ethanol production has been suggested as a Liquefaction lower cost option in future bioreﬁneries. Here, we propose a closed-loop strategy to produce the cellulolytic Cellulases enzymes required for biomass hydrolysis using sugarcane bagasse liqueﬁed by a home-made enzymatic cocktail Enzymes as carbon source and inducer. The fed-batch liquefaction conditions were ﬁrstly evaluated using commercial Bioreﬁnery enzymes. Subsequently, the eﬀects of diﬀerent liqueﬁed materials and solids loadings on endoglucanase pro- Biomass hydrolysis duction by Aspergillus niger cultivated in submerged fermentation were investigated. The liqueﬁed bagasse produced using the home-made cocktail was more favorable for endoglucanase production, resulting in im- provement up to 17%, compared to bagasse liqueﬁed by
Bioresource Technology – Elsevier
Published: Feb 1, 2018
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