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Rapid detection and serotyping of adenovirus by direct immunofluorescence

Rapid detection and serotyping of adenovirus by direct immunofluorescence Four fluorescent antibody reagents were evaluated for their suitability for the identification of adenovirus isolates by immunofluorescence. The antibodies used in the reagents consist of monoclonal antibodies against adenovirus type 3 (Ad3), Ad4, Ad8, and adenoviruses of subgroup C (Ad1,2,5,6), serotypes known to occur in outbreaks of disease. Most of the monoclonal antibodies employed were reactive against type‐specific antigens found on the hexon protein. Reagents employing two noncompeting anti‐hexon antibodies were more sensitive than reagents prepared with only one monoclonal antibody, although both types of reagents exhibited a high degree of specificity. Five hundred and seventeen adenovirus isolates (359 of which had previously been typed by other methods) and 46 nonadenovirus isolates were examined with all four type‐specific reagents in parallel with an adenovirus group‐specific reagent. The results indicate that direct typing of adenovirus isolates is feasible, leading to significant savings in time compared to other typing methods and should contribute to the management of certain adenovirus infections, particularly during outbreaks. J. Med. Virol. 51:198–201, 1997. © 1997 Wiley‐Liss, Inc. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Medical Virology Wiley

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References (23)

Publisher
Wiley
Copyright
Copyright © 1997 Wiley‐Liss, Inc.
ISSN
0146-6615
eISSN
1096-9071
DOI
10.1002/(SICI)1096-9071(199703)51:3<198::AID-JMV9>3.0.CO;2-1
Publisher site
See Article on Publisher Site

Abstract

Four fluorescent antibody reagents were evaluated for their suitability for the identification of adenovirus isolates by immunofluorescence. The antibodies used in the reagents consist of monoclonal antibodies against adenovirus type 3 (Ad3), Ad4, Ad8, and adenoviruses of subgroup C (Ad1,2,5,6), serotypes known to occur in outbreaks of disease. Most of the monoclonal antibodies employed were reactive against type‐specific antigens found on the hexon protein. Reagents employing two noncompeting anti‐hexon antibodies were more sensitive than reagents prepared with only one monoclonal antibody, although both types of reagents exhibited a high degree of specificity. Five hundred and seventeen adenovirus isolates (359 of which had previously been typed by other methods) and 46 nonadenovirus isolates were examined with all four type‐specific reagents in parallel with an adenovirus group‐specific reagent. The results indicate that direct typing of adenovirus isolates is feasible, leading to significant savings in time compared to other typing methods and should contribute to the management of certain adenovirus infections, particularly during outbreaks. J. Med. Virol. 51:198–201, 1997. © 1997 Wiley‐Liss, Inc.

Journal

Journal of Medical VirologyWiley

Published: Mar 1, 1997

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